Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Myeloblastin (PRTN3) Standard Curve.)
Mouse Myeloblastin (PRTN3) ELISA Kit | MBT elisa kit
Mouse Myeloblastin (PRTN3) ELISA Kit
Gene Names
Prtn3; PR3; mPR3
Reactivity
Mouse
Synonyms
Myeloblastin (PRTN3); Mouse Myeloblastin (PRTN3) ELISA Kit; PRTN3; ACPA; AGP7; C-ANCA; CANCA; MBN; NP4; P29; PR-3; PR3; Wegener granulomatosis autoantigen; azurophil granule protein 7; leukocyte proteinase 3; myeloblastin; serine proteinase; neutrophil; MBT elisa kit
Reactivity
Mouse
Specificity
Mouse Myeloblastin (PRTN3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PRTN3. No significant cross-reactivity or interference between Mouse PRTN3 and analogues was observed.
Sequence Length
254
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Myeloblastin (PRTN3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Myeloblastin (PRTN3) Standard Curve.)
Related Product Information for MBT elisa kit
Description: This Mouse Myeloblastin (PRTN3) ELISA Kit employs a two-site sandwich ELISA to quantitate PRTN3 in samples. An antibody specific for PRTN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRTN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRTN3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRTN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PRTN3 is a serine protease enzyme expressed mainly in neutrophil granulocytes. Its exact role in the function of the neutrophil is unknown but in human neutrophils proteinase 3 contributes to the proteolytic generation of antimicrobial peptides. It is also the epitope of anti-neutrophil cytoplasmic antibodies (ANCAs) of the cANCA (cytoplasmic subtype) class, a type of antibody frequently found in the disease Wegener's granulomatosis. Proteinase K is commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification.
Background: PRTN3 is a serine protease enzyme expressed mainly in neutrophil granulocytes. Its exact role in the function of the neutrophil is unknown but in human neutrophils proteinase 3 contributes to the proteolytic generation of antimicrobial peptides. It is also the epitope of anti-neutrophil cytoplasmic antibodies (ANCAs) of the cANCA (cytoplasmic subtype) class, a type of antibody frequently found in the disease Wegener's granulomatosis. Proteinase K is commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
myeloblastin
NCBI Official Synonym Full Names
proteinase 3
NCBI Official Symbol
Prtn3
NCBI Official Synonym Symbols
PR3; mPR3
NCBI Protein Information
myeloblastin
UniProt Protein Name
Myeloblastin
Protein Family
UniProt Gene Name
Prtn3
UniProt Synonym Gene Names
PR-3
Uniprot Description
Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters.
Research Articles on MBT
Similar Products
Product Notes
The Mouse MBT prtn3 (Catalog #AAA9717239) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717239 ELISA Kit recognizes Mouse MBT. It is sometimes possible for the material contained within the vial of "Myeloblastin (PRTN3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
