Rabbit BAG6 / G3 / Scythe Polyclonal Antibody | anti-BAG6 antibody

Rabbit Polyclonal to Mouse BAG6 / G3 / Scythe

Cat. Num. AAA243386

• Gene Names: BAG6; G3; BAT3; BAG-6; D6S52E
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Unpurified serum
• Synonyms: BAG6 / G3 / Scythe; Polyclonal Antibody; Rabbit Polyclonal to Mouse BAG6 / G3 / Scythe; Anti-BAG6 / G3 / Scythe Antibody (C-Terminus) IHC-plus; BAG6; BCL2-associated athanogene 6; D6S52E; HLA-B associated transcript 3; HLA-B-associated transcript 3; HLA-B associated transcript-3; G3; Protein G3; Protein Scythe; Scythe; BAG-6; BAT3; Mouse BAG6; anti-BAG6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous Scythe/BAT3 in cell lysates.
• Purity/Purification: Unpurified serum
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
• Concentration: 80 mg/ml (varies by lot)
• Sequence Length: 1132

• Applicable Applications for anti-BAG6 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 - 10 ug/ml), WB (1:5000 - 1:8000); Usage: This polyclonal antibody has been tested for use in western blotting and is specific for mouse Scythe/Bat3 and its isoforms. Specific conditions for reactivity should be optimized by the end user. Expect multiple bands, with the predominant at approx...
• Target Species: Mouse
• Immunogen Description: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal portion of mouse Scythe/BAT3.
• Immunogen Type: Synthetic peptide
• Immunogen: BAG6 / G3 / Scythe antibody was raised against this antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal portion of mouse Scythe/BAT3.
• Antigen Modification: C-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-BAG6 / G3 / Scythe antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5-10 ug/ml.)

Western Blot (WB)

(Western Blot showing detection of mouse Scythe/BAT3 by anti-Mouse Scythe/BAT3 antibody in various tissues of mouse WT and Scythe knockout (-/-) embryos.)

NCBI and Uniprot Product Information

• NCBI GI #: 149158692
• NCBI GeneID: 7917
• NCBI Accession #: NP_004630.3
• NCBI GenBank Nucleotide #: NM_004639.3
• UniProt Accession #: P46379; O95874; A2ADJ7; A3KQ42; A3KQ44; A6NGY6; A6PWF7; B0UX84; B4DZ12; B4E3V4; E7EMZ4; F8VXY4
• Molecular Weight: 113,456 Da
• NCBI Official Full Name: large proline-rich protein BAG6 isoform a
• NCBI Official Synonym Full Names: BCL2-associated athanogene 6
• NCBI Official Symbol: BAG6
• NCBI Official Synonym Symbols: G3; BAT3; BAG-6; D6S52E
• NCBI Protein Information: large proline-rich protein BAG6; scythe; protein G3; protein Scythe; HLA-B associated transcript 3; HLA-B associated transcript-3; HLA-B-associated transcript 3; large proline-rich protein BAT3; BAG family molecular chaperone regulator 6
• UniProt Protein Name: Large proline-rich protein BAG6
• Protein Family: BAG family molecular chaperone regulator
• UniProt Gene Name: BAG6
• UniProt Synonym Gene Names: BAT3; G3; BAG-6; BAG6
• UniProt Entry Name: BAG6_HUMAN

NCBI Description

This gene was first characterized as part of a cluster of genes located within the human major histocompatibility complex class III region. This gene encodes a nuclear protein that is cleaved by caspase 3 and is implicated in the control of apoptosis. In addition, the protein forms a complex with E1A binding protein p300 and is required for the acetylation of p53 in response to DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

BAT3: Chaperone that plays a key role in various processes such as apoptosis, insertion of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane and regulation of chromatin. Acts in part by regulating stability of proteins and their degradation by the proteasome. Participates in endoplasmic reticulum stress-induced apoptosis via its interaction with AIFM1/AIF by regulating AIFM1/AIF stability and preventing its degradation. Also required during spermatogenesis for synaptonemal complex assembly via its interaction with HSPA2, by inhibiting polyubiquitination and subsequent proteasomal degradation of HSPA2. Required for selective ubiquitin-mediated degradation of defective nascent chain polypeptides by the proteasome. In this context, may play a role in immuno-proteasomes to generate antigenic peptides via targeted degradation, thereby playing a role in antigen presentation in immune response. Key component of the BAG6/BAT3 complex, a cytosolic multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. BAG6/BAT3 acts by facilitating TA membrane proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins and transfers them to ASNA1/TRC40 for targeting to the endoplasmic reticulum membrane. Component of the BAT3 complex, at least composed of BAG6/BAT3, UBL4A and GET3/TRC35. Interacts with AIFM1, CTCFL, HSPA2 and p300/EP300. Interacts with ricin A chain. Interacts with L.pneumophila proteins Lpg2160 and LegU1. Interacts with NCR3. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Apoptosis; Cell cycle regulation; Chaperone

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; ubiquitin protein ligase binding; ribosome binding; Hsp70 protein binding; polyubiquitin binding

Biological Process: ubiquitin-dependent protein catabolic process; negative regulation of proteolysis; synaptonemal complex assembly; internal peptidyl-lysine acetylation; protein stabilization; immune system process; chromatin modification; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; regulation of cell proliferation; embryonic development; transport; spermatogenesis; brain development; kidney development; cell differentiation; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; lung development; negative regulation of apoptosis