Rabbit anti-Human BAG4 Polyclonal Antibody | anti-BAG4 antibody

BAG4, CT (BAG4, SODD, BAG family molecular chaperone regulator 4, Bcl-2-associated athanogene 4, Silencer of death domains) (Azide free) (HRP)

Cat. Num. AAA6277541

• Gene Names: BAG4; SODD; BAG-4
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BAG4; Polyclonal Antibody; CT (BAG4; SODD; BAG family molecular chaperone regulator 4; Bcl-2-associated athanogene 4; Silencer of death domains) (Azide free) (HRP); Silencer of death domains; anti-BAG4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-BAG4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: BAG4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-409 amino acids from the C-terminal region of human BAG4.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(BAG4 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the BAG4 antibody detected the BAG4 protein (arrow).)

Related Product Information for anti-BAG4 antibody

BAG4 is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. This protein was found to be associated with the death domain of tumor necrosis factor receptor type 1 (TNF-R1) and death receptor-3 (DR3), and thereby negatively regulates downstream cell death signaling. The regulatory role of this protein in cell death was demonstrated in epithelial cells which undergo apoptosis while integrin mediated matrix contacts are lost.

Product Categories/Family for anti-BAG4 antibody

Antibodies; Apoptosis

References

Bailey, S.D., et al. Diabetes Care (2010). In press: Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Tao, H.F., et al. Zhongguo Shi Yan Xue Ye Xue Za Zhi 15(3):501-505(2007). Riley, B.M., et al. Am. J. Med. Genet. A 143A (8), 846-852 (2007): Yang, Z.Q., et al. Cancer Res. 66(24):11632-11643(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 6631075
• NCBI GeneID: 9530
• NCBI Accession #: NP_004865.1
• NCBI GenBank Nucleotide #: NM_004874.3
• UniProt Accession #: O95429
• NCBI Official Full Name: BAG family molecular chaperone regulator 4 isoform 1
• NCBI Official Synonym Full Names: BCL2 associated athanogene 4
• NCBI Official Symbol: BAG4
• NCBI Official Synonym Symbols: SODD; BAG-4
• NCBI Protein Information: BAG family molecular chaperone regulator 4
• UniProt Protein Name: BAG family molecular chaperone regulator 4
• Protein Family: BAG family molecular chaperone regulator
• UniProt Gene Name: BAG4
• UniProt Synonym Gene Names: SODD; BAG-4
• UniProt Entry Name: BAG4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. This protein was found to be associated with the death domain of tumor necrosis factor receptor type 1 (TNF-R1) and death receptor-3 (DR3), and thereby negatively regulates downstream cell death signaling. The regulatory role of this protein in cell death was demonstrated in epithelial cells which undergo apoptosis while integrin mediated matrix contacts are lost. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2011]

Uniprot Description

BAG4: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Prevents constitutive TNFRSF1A signaling. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 8p11.23

Cellular Component: plasma membrane; nucleus; cytosol

Molecular Function: protein binding; chaperone binding; receptor signaling protein activity

Biological Process: positive regulation of protein kinase B signaling cascade; positive regulation of cell adhesion; protein folding; positive regulation of actin filament polymerization; protein heterooligomerization; positive regulation of stress fiber formation; positive regulation of peptidyl-serine phosphorylation; negative regulation of apoptosis