Rabbit anti-Human BAG3 Polyclonal Antibody | anti-BAG3 antibody

Anti-BAG3 Antibody, Rabbit Polyclonal

Cat. Num. AAA8103593

• Gene Names: BAG3; BIS; BAG-3; CAIR-1
• Reactivity: Human
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: BAG3; Polyclonal Antibody; Anti-BAG3 Antibody; Rabbit Polyclonal; BAG3 Antibody; Rabbit PAb; Antigen Affinity Purified; Bcl2-associated athanogene 3; Anti-BAG-3 Antibody; Anti-BIS Antibody; Anti-CAIR-1 Antibody; Anti-MFM6 Antibody; anti-BAG3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human BAG3
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-BAG3 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human BAG3
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human BAG3, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-BAG3 rabbit polyclonal antibody at 1:500 dilution Lane A: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:62 kDa Observed band size:80 kDa)

Related Product Information for anti-BAG3 antibody

Bcl2-associated athanogene 3 (BAG3) is a 575 amino acid protein that is found predominantly in the heart, skeletal muscle, and many cancers. Deletions and truncations in BAG3 that result in haplo-insufficiency have been associated with the development of dilated cardiomyopathy. BAG3 is a stress-induced molecular co-chaperone that functions to regulate cellular protein homeostasis and cell survival via Chaperone-Assisted Selective Autophagy (CASA). CASA and specifically BAG3 may represent a novel host defense mechanism, whereby BAG3 may dampen egress of several hemorrhagic fever viruses by interacting and interfering with the budding function of viral PPxY-containing matrix proteins. BCL2-associated athanogene (BAG)-3 is viewed as a platform that would physically and functionally link distinct classes of molecular chaperones of the heat shock protein (HSP) family for the stabilization and clearance of damaged proteins. The HSPB8-BAG3 connection in mounting of an efficient stress response, which may be involved in BAG3-related human diseases. Both Bcl-2-associated athanogene 3 (BAG3) and tumor necrosis factor-related apopt-osis inducing ligand (TRAIL) involved in apoptosis.

NCBI and Uniprot Product Information

• NCBI GI #: 14043024
• NCBI GeneID: 9531
• NCBI Accession #: NP_004272.2
• NCBI GenBank Nucleotide #: NM_004281.3
• UniProt Accession #: O95817; Q3B763; Q9NT20; Q9P120; A8K5L8
• Molecular Weight: 61,595 Da
• NCBI Official Full Name: BAG family molecular chaperone regulator 3
• NCBI Official Synonym Full Names: BCL2-associated athanogene 3
• NCBI Official Symbol: BAG3
• NCBI Official Synonym Symbols: BIS; BAG-3; CAIR-1
• NCBI Protein Information: BAG family molecular chaperone regulator 3; docking protein CAIR-1; BCL2-binding athanogene 3; bcl-2-binding protein Bis
• UniProt Protein Name: BAG family molecular chaperone regulator 3
• Protein Family: BAG family molecular chaperone regulator
• UniProt Gene Name: BAG3
• UniProt Synonym Gene Names: BIS; BAG-3
• UniProt Entry Name: BAG3_HUMAN

NCBI Description

BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008]

Uniprot Description

BAG3: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity. Defects in BAG3 are the cause of myopathy myofibrillar type 6 (MFM6). A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material. Defects in BAG3 are the cause of cardiomyopathy dilated type 1HH (CMD1HH). CMD1HH is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 10q25.2-q26.2

Cellular Component: neuron projection; cytoplasm; plasma membrane; cytosol; Z disc

Molecular Function: protein binding; chaperone binding; protein complex binding

Biological Process: induction of apoptosis via death domain receptors; protein folding; protein stabilization; spinal cord development; brain development; negative regulation of apoptosis

Disease: Myopathy, Myofibrillar, 6; Cardiomyopathy, Dilated, 1hh