anti-Human, Rat Bag3 Polyclonal Antibody | anti-BAG3 antibody

Anti-Bag3 Antibody

Cat. Num. AAA178007

• Gene Names: BAG3; BIS; MFM6; BAG-3; CAIR-1
• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: Bag3; Polyclonal Antibody; Anti-Bag3 Antibody; BAG family molecular chaperone regulator 3; BAG 3; BAG-3; BAG3_HUMAN; Bcl 2 binding protein; Bcl-2-associated athanogene 3; Bcl-2-binding protein Bis; BCL2 associated athanogene 3; BCL2 binding athanogene 3; BIS; Docking protein CAIR 1; Docking protein CAIR-1 antibody; BCL2-associated athanogene 3; anti-BAG3 antibody

• Reactivity: Human, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 575

• Applicable Applications for anti-BAG3 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: E Coli-derived human Bag3 recombinant protein (Position: H100-N561). Human Bag3 shares 84.1% amino acid (aa) sequence identity with mouse Bag3.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- Bag3 Picoband antibody, MBS178007, Western blottingAll lanes: Anti Bag3 (MBS178007) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Rat Testis Tissue Lysate at 50ugLane 3: MCF-7 Whole Cell Lysate at 40ugLane 4: HELA Whole Cell Lysate at 40ugPredicted bind size: 62KDObserved bind size: 62KD )

Immunohistochemistry (IHC)

(Anti- Bag3 Picoband antibody, MBS178007, IHC(P)IHC(P): Human Lung Cancer Tissue )

Related Product Information for anti-BAG3 antibody

Description: Rabbit IgG polyclonal antibody for BAG family molecular chaperone regulator 3(BAG3) detection. Tested with WB, IHC-P in Human;Rat.

Background: BAG family molecular chaperone regulator 3(BAG3) is a member of a conserved family of cyto-protective proteins that bind to and regulate Hsp70 family molecular chaperones. BAG3 mutations are responsible for familial dilated cardiomyopathy. BAG3 polymorphisms are also associated with sporadic forms of the disease together with HSPB7 locus. In muscle cells, BAG3 cooperates with the molecular chaperones Hsc70 and HspB8 to induce the degradation of mechanically damaged cytoskeleton components in lysosomes. This process is called chaperone-assisted selective autophagy (CASA) and is essential for maintaining muscle activity in flies, mice and men.

References

1. Arndt V, Dick N, Tawo R, Dreiseidler M, Wenzel D, Hesse M, Fürst DO, Saftig P, Saint R, Fleischmann BK, Hoch M, Höhfeld J (January 2010). "Chaperone-assisted selective autophagy is essential for muscle maintenance". Curr Biol 20 (2): 143-8. 2. Homma, S., Iwasaki, M., Shelton, G. D., Engvall, E., Reed, J. C., Takayama, S. BAG3 deficiency results in fulminant myopathy and early lethality. Am. J. Path. 169: 761-773, 2006. 3. Villard, E; Perret, C, Gary, F, Proust, C, Dilanian, G, Hengstenberg, C, Ruppert, V, Arbustini, E, Wichter, T, Germain, M, Dubourg, O, Tavazzi, L, Aumont, MC, DeGroote, P, Fauchier, L, Trochu, JN, Gibelin, P, Aupetit, JF, Stark, K, Erdmann, J, Hetzer, R, Roberts, AM, Barton, PJ, Regitz-Zagrosek, V; Cardiogenics Consortium, Aslam, U, Duboscq-Bidot, L, Meyborg, M, Maisch, B, Madeira, H, Waldenström, A, Galve, E, Cleland, JG, Dorent, R, Roizes, G, Zeller, T, Blankenberg, S, Goodall, AH, Cook, S, Tregouet, DA, Tiret, L, Isnard, R, Komajda, M, Charron, P, Cambien, F (April 1, 2011). A genome-wide association study identifies two loci associated with heart failure due to dilated cardiomyopathy 32 (9). pp. 1065-76.

NCBI and Uniprot Product Information

• NCBI GI #: 14043024
• NCBI GeneID: 9531
• NCBI Accession #: NP_004272.2
• NCBI GenBank Nucleotide #: NM_004281.3
• UniProt Accession #: O95817; Q3B763; Q9NT20; Q9P120; A8K5L8
• Molecular Weight: 61,595 Da
• NCBI Official Full Name: BAG family molecular chaperone regulator 3
• NCBI Official Synonym Full Names: BCL2 associated athanogene 3
• NCBI Official Symbol: BAG3
• NCBI Official Synonym Symbols: BIS; MFM6; BAG-3; CAIR-1
• NCBI Protein Information: BAG family molecular chaperone regulator 3
• UniProt Protein Name: BAG family molecular chaperone regulator 3
• Protein Family: BAG family molecular chaperone regulator
• UniProt Gene Name: BAG3
• UniProt Synonym Gene Names: BIS; BAG-3
• UniProt Entry Name: BAG3_HUMAN

NCBI Description

BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008]

Uniprot Description

BAG3: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity. Defects in BAG3 are the cause of myopathy myofibrillar type 6 (MFM6). A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material. Defects in BAG3 are the cause of cardiomyopathy dilated type 1HH (CMD1HH). CMD1HH is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 10q25.2-q26.2

Cellular Component: cytoplasm; cytosol; neuron projection; plasma membrane; Z disc

Molecular Function: adenyl-nucleotide exchange factor activity; chaperone binding; protein binding; protein complex binding

Biological Process: brain development; induction of apoptosis via death domain receptors; negative regulation of apoptosis; protein folding; protein stabilization; regulation of catalytic activity; spinal cord development

Disease: Cardiomyopathy, Dilated, 1hh; Myopathy, Myofibrillar, 6