Rabbit anti-Human BAG2 Polyclonal Antibody | anti-BAG2 antibody

BAG2, CT (BAG2, BAG family molecular chaperone regulator 2, Bcl-2-associated athanogene 2) (Azide free) (HRP)

Cat. Num. AAA6277530

• Gene Names: BAG2; BAG-2; dJ417I1.2
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BAG2; Polyclonal Antibody; CT (BAG2; BAG family molecular chaperone regulator 2; Bcl-2-associated athanogene 2) (Azide free) (HRP); Bcl-2-associated athanogene 2; anti-BAG2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BAG2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-BAG2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 148-176 in the C-terminal region of human BAG2, UniProt Accession #Q95816.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in NCI-H460 cell line lysates (35ug/lane) usingMBS644807.This demonstrates the BAG2 antibody detected the BAG2 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human ovarian carcinoma using MBS644807 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Related Product Information for anti-BAG2 antibody

BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq].

Product Categories/Family for anti-BAG2 antibody

Antibodies; Apoptosis

References

Arndt, V., et al. Mol. Biol. Cell 16(12):5891-5900(2005). Dai, Q., et al. J. Biol. Chem. 280(46):38673-38681(2005). Ueda, K., et al. J. Biol. Chem. 279(40):41815-41821(2004). Ueda, K., et al. J. Biol. Chem. 279(40):41815-41821(2004). Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 4757834
• NCBI GeneID: 9532
• NCBI Accession #: NP_004273.1
• NCBI GenBank Nucleotide #: NM_004282.3
• UniProt Accession #: O95816; Q08AS9; Q6FID0; B4DXE2
• Molecular Weight: 20,189 Da
• NCBI Official Full Name: BAG family molecular chaperone regulator 2
• NCBI Official Synonym Full Names: BCL2-associated athanogene 2
• NCBI Official Symbol: BAG2
• NCBI Official Synonym Symbols: BAG-2; dJ417I1.2
• NCBI Protein Information: BAG family molecular chaperone regulator 2; BAG-family molecular chaperone regulator-2; bcl-2-associated athanogene 2; dJ417I1.2 (BAG-family molecular chaperone regulator 2)
• UniProt Protein Name: BAG family molecular chaperone regulator 2
• Protein Family: BAG family molecular chaperone regulator
• UniProt Gene Name: BAG2
• UniProt Synonym Gene Names: BAG-2
• UniProt Entry Name: BAG2_HUMAN

NCBI Description

BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008]

Uniprot Description

BAG2: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 6p12.1-p11.2

Molecular Function: identical protein binding; protein binding; chaperone binding

Biological Process: protein folding; protein metabolic process