Typical Testing Data/Standard Curve (for reference only)
Rat Cyclooxygenase-1 (COX-1) ELISA Kit | COX-1 elisa kit
Rat Cyclooxygenase-1 (COX-1) ELISA Kit
Gene Names
PTGS1; COX-1
Reactivity
Rat
Synonyms
Cyclooxygenase-1 (COX-1); Rat Cyclooxygenase-1 (COX-1) ELISA Kit; PTGS1; RP11-542K23.6; COX1; COX3; PCOX1; PGG/HS; PGHS-1; PGHS1; PHS1; PTGHS; prostaglandin G/H synthase and cyclooxygenase; prostaglandin-endoperoxide synthase 1; COX-1 elisa kit
Reactivity
Rat
Sequence Length
606
Assay Type
Sandwich
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for COX-1 elisa kit
Principle of the Assay: Rat Cyclooxygenase-1 (COX-1) ELISA Kit employs a two-site sandwich ELISA to quantitate COX-1 in samples. An antibody specific for COX-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any COX-1 present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Rat COX-1 detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of COX-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background/Introduction: Cyclooxygenase (COX), first purified in 1976 and cloned in 1988, is the key enzyme in the synthesis of prostaglandins (PGs) from arachidonic acid. The two isoforms of COX are almost identical in structure but have important differences in substrate and inhibitor selectivity and in their intracellular locations. Protective PGs, which preserve the integrity of the stomach lining and maintain normal renal function in a compromised kidney, are synthesized by COX-1. COX-1 is constitutively expressed in most tissues (e.g., platelets, lung, prostate, brain, gastrointestinal tract, kidney, liver and spleen), where the COX-1-derived prostanoids are involved in homeostatic functions.
Background/Introduction: Cyclooxygenase (COX), first purified in 1976 and cloned in 1988, is the key enzyme in the synthesis of prostaglandins (PGs) from arachidonic acid. The two isoforms of COX are almost identical in structure but have important differences in substrate and inhibitor selectivity and in their intracellular locations. Protective PGs, which preserve the integrity of the stomach lining and maintain normal renal function in a compromised kidney, are synthesized by COX-1. COX-1 is constitutively expressed in most tissues (e.g., platelets, lung, prostate, brain, gastrointestinal tract, kidney, liver and spleen), where the COX-1-derived prostanoids are involved in homeostatic functions.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
prostaglandin G/H synthase 1
NCBI Official Symbol
PTGS1
NCBI Official Synonym Symbols
COX-1
NCBI Protein Information
prostaglandin G/H synthase 1
UniProt Protein Name
Prostaglandin G/H synthase 1
UniProt Gene Name
PTGS1
UniProt Synonym Gene Names
COX1; COX-1; PGH synthase 1; PGHS-1; PHS 1
UniProt Entry Name
PGH1_RABIT
Uniprot Description
Function: Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells
Catalytic activity: Arachidonate + AH2 + 2 O2 = prostaglandin H2 + A + H2O.
Cofactor: Binds 1 heme B (iron-protoporphyrin IX) group per subunit
Research Articles on COX-1
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Product Notes
The Rat COX-1 ptgs1 (Catalog #AAA9712025) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712025 ELISA Kit recognizes Rat COX-1. It is sometimes possible for the material contained within the vial of "Cyclooxygenase-1 (COX-1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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