Rabbit anti-Mouse BAG1 Polyclonal Antibody | anti-BAG1 antibody

RABBIT ANTI BAG1 (N-TERMINAL)

Cat. Num. AAA221460

• Gene Names: BAG1; HAP; BAG-1; RAP46
• Reactivity: Mouse
• Applications: Western Blot
• Synonyms: BAG1; Polyclonal Antibody; RABBIT ANTI BAG1 (N-TERMINAL); anti-BAG1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item detects an epitope within the N-terminal (NT) region of Bcl-2 associated athanogene-1 (BAG1), a co-chaperone protein which binds to and regulates the 70kD heat shock proteins (Hsp70/Hsc70). BAG1 has also been shown to interact with the kinase Raf1, a number of steroid and tyrosine kinase receptors, and the Bcl-2 family of proteins. It is thought to function as a molecular switch between cell growth and differentiation, and cell survival. ; ; The BAG1 gene encodes three different isoforms; a predominantly cytosolic form of 36kD (BAG1S), a 46kD cytosolic and nuclear form (BAG1M), and a predominantly nuclear form 50kD (BAG1L). ; ; BAG1 displays potent neuroprotective activity in vivo against stroke, and could be used for reducing brain injury during cerebral ischemia and neurodegenerative diseases. BAG1 may also promote chemoresistance, growth factor independence, and anchorage independent cell growth in tumour cells.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 230

• Applicable Applications for anti-BAG1 antibody: Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 35kDa in PC-3 cell lysate.; Western Blotting: Minimum Dilution: 1; Maximum Dilution: 2ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide
• Immunogen: 14 amino acid peptide near the amino terminus of human BAG1.; Buffer Solution
• Antiserum Preparation: Antisera to BAG1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of whole cell lysate from PC-3 human prostate cancer cells probed with Rabbit anti Human BAG1 (MBS221460) at 1(A) and 2(B) ug/ml)

Testing Data #2

(Immunohistochemical staining of human lymph node with Rabbit anti Human BAG1 (MBS221460))

Related Product Information for anti-BAG1 antibody

MBS221460 detects an epitope within the N-terminal (NT) region of Bcl-2 associated athanogene-1 (BAG1), a co-chaperone protein which binds to and regulates the 70kD heat shock proteins (Hsp70/Hsc70). BAG1 has also been shown to interact with the kinase Raf1, a number of steroid and tyrosine kinase receptors, and the Bcl-2 family of proteins. It is thought to function as a molecular switch between cell growth and differentiation, and cell survival. The BAG1 gene encodes three different isoforms; a predominantly cytosolic form of 36kD (BAG1S), a 46kD cytosolic and nuclear form (BAG1M), and a predominantly nuclear form 50kD (BAG1L). BAG1 displays potent neuroprotective activity in vivo against stroke, and could be used for reducing brain injury during cerebral ischemia and neurodegenerative diseases. BAG1 may also promote chemoresistance, growth factor independence, and anchorage independent cell growth in tumour cells.

NCBI and Uniprot Product Information

• NCBI GI #: 288915527
• NCBI GeneID: 573
• NCBI Accession #: NP_001165886.1
• NCBI GenBank Nucleotide #: NM_001172415.1
• UniProt Accession #: Q99933; O75315; Q14414; Q53H32; Q5VZE8; Q5VZE9; Q5VZF0; Q96TG2; Q9Y2V4
• Molecular Weight: 25,989 Da
• NCBI Official Full Name: BAG family molecular chaperone regulator 1 isoform BAG-1S
• NCBI Official Synonym Full Names: BCL2-associated athanogene
• NCBI Official Symbol: BAG1
• NCBI Official Synonym Symbols: HAP; BAG-1; RAP46
• NCBI Protein Information: BAG family molecular chaperone regulator 1; Bcl-2 associating athanogene-1 protein; Bcl-2-binding protein; glucocortoid receptor-associated protein RAP46; receptor-associated protein, 46-KD
• UniProt Protein Name: BAG family molecular chaperone regulator 1
• Protein Family: BAG family molecular chaperone regulator
• UniProt Gene Name: BAG1
• UniProt Synonym Gene Names: HAP; BAG-1
• UniProt Entry Name: BAG1_HUMAN

NCBI Description

The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. Multiple protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) initiation codon, and three alternative downstream AUG initiation codons. A related pseudogene has been defined on chromosome X. [provided by RefSeq, Feb 2010]

Uniprot Description

BAG1: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli. Homodimer. Forms a heteromeric complex with HSP70/HSC70. Binds to the ATPase domain of HSP/HSC70 chaperones. Isoform 1, isoform 3 and isoform 4 but not isoform 2 interact with HSPA8/HSC70. Interacts with NR3C1. Interacts with the N-terminal region of STK19. Interacts with PPP1R15A. Interacts with BCL2 in an ATP-dependent manner. Isoform 2 does not interact with BCL2. Up-regulated during differentiation of bladder epithelial cells and down-regulated during differentiation of prostate epithelium. Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 9p12

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; chaperone binding; receptor signaling protein activity

Biological Process: cell surface receptor linked signal transduction; apoptosis; negative regulation of apoptosis