Rabbit Bad Polyclonal Antibody

Bad, phosphorylated (Ser75) (Bcl2 Antagonist of Cell Death)

Cat. Num. AAA630118

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography followed by IAP.
• Synonyms: Bad; Polyclonal Antibody; phosphorylated (Ser75) (Bcl2 Antagonist of Cell Death); Anti -Bad; anti-Bad antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes the 18kD human Phospho-Bad-S75 protein. Species Crossreactivity: mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography followed by IAP.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-Bad antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:250
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding S75 of human Bad (KLH). Cellular Localization: Mitochondrion outer membrane. Cytoplasm. Epitope: pSer75
• Positive Control: RAW264.7/LPS lysate.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot Analysis: RAW264.7 untreated (lane 1),LPS treated (lane 2) lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with the Ab (1:250 dilution of this lot). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein p Bad (~18kD).)

Related Product Information for anti-Bad antibody

Bad is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin are found to be involved in the regulation of this protein. Bad is phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation.

Product Categories/Family for anti-Bad antibody

Antibodies; Abs to Apoptosis