Western Blot (WB)
(Western blot analysis of DHHC-9 using COLO205 whole cell lysates)
Rabbit DHHC-9 Polyclonal Antibody | anti-DHHC-9 antibody
DHHC-9 Antibody
Gene Names
ZDHHC9; CGI89; DHHC9; MMSA1; MRXSZ; ZNF379; ZNF380; CXorf11; ZDHHC10
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin
Synonyms
DHHC-9; Polyclonal Antibody; DHHC-9 Antibody; Antigen MMSA 1; Asp His His Cys domain containing protein 9; CGI89; CXorf11; DHHC9; MMSA1; MRXSZ; Palmitoyltransferase ZDHHC9; ZDHC9_HUMAN; ZDHHC 9; ZDHHC10; ZDHHC9; Zinc finger DHHC domain containing 10; Zinc finger DHHC domain containing protein 9; Zinc finger DHHC domain-containing protein 9; Zinc finger DHHC type containing 9; Zinc finger protein 379; Zinc finger protein 380; ZNF379; ZNF380; anti-DHHC-9 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
DHHC-9 antibody detects endogenous levels of total DHHC-9
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin
Form/Format
Liquid
Phosphate buffered saline
Phosphate buffered saline
Concentration
1mg/ml (varies by lot)
Sequence Length
364
Applicable Applications for anti-DHHC-9 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:1000
IF/ICC: 1:100-1:500
ELISA (EIA) (peptide): 1:20000-1:40000
The optimal dilutions should be determined by the end user.
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
IF/ICC: 1:100-1:500
ELISA (EIA) (peptide): 1:20000-1:40000
The optimal dilutions should be determined by the end user.
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Immunogen
A synthesized peptide derived from human DHHC-9, corresponding to a region within C-terminal amino acids.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of DHHC-9 using COLO205 whole cell lysates)
Western Blot (WB)
(Western blot analysis of DHHC-9 using COLO205 whole cell lysates)
Immunofluorescene (IF)
(MBS9602097 staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9602097 staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41kDa
NCBI Official Full Name
palmitoyltransferase ZDHHC9
NCBI Official Synonym Full Names
zinc finger DHHC-type containing 9
NCBI Official Symbol
ZDHHC9
NCBI Official Synonym Symbols
CGI89; DHHC9; MMSA1; MRXSZ; ZNF379; ZNF380; CXorf11; ZDHHC10
NCBI Protein Information
palmitoyltransferase ZDHHC9
UniProt Protein Name
Palmitoyltransferase ZDHHC9
UniProt Gene Name
ZDHHC9
UniProt Synonym Gene Names
CXorf11; ZDHHC10; ZNF379; ZNF380; DHHC-9; DHHC9
NCBI Description
This gene encodes an integral membrane protein that is a member of the zinc finger DHHC domain-containing protein family. The encoded protein forms a complex with golgin subfamily A member 7 and functions as a palmitoyltransferase. This protein specifically palmitoylates HRAS and NRAS. Mutations in this gene are associated with X-linked cognitive disability. Alternate splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, May 2010]
Uniprot Description
The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS.
Research Articles on DHHC-9
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Product Notes
The DHHC-9 zdhhc9 (Catalog #AAA9602097) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DHHC-9 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DHHC-9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:1000 IF/ICC: 1:100-1:500 ELISA (EIA) (peptide): 1:20000-1:40000 The optimal dilutions should be determined by the end user. IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. . Researchers should empirically determine the suitability of the DHHC-9 zdhhc9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DHHC-9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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