Rabbit anti-Human BAD Polyclonal Antibody | anti-BAD antibody

BAD, Phosphorylated (S124) (BAD, BBC6, BCL2L8, Bcl2 antagonist of cell death, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl-XL/Bcl-2-associated death promoter) (PE)

Cat. Num. AAA6277338

• Gene Names: BAD; BBC2; BCL2L8
• Reactivity: Human
• Applications: Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: BAD; Polyclonal Antibody; Phosphorylated (S124) (BAD; BBC6; BCL2L8; Bcl2 antagonist of cell death; Bcl-2-binding component 6; Bcl-2-like protein 8; Bcl-XL/Bcl-2-associated death promoter) (PE); Bcl-XL/Bcl-2-associated death promoter; anti-BAD antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-BAD antibody: FLISA, Dot Blot (DB)
• Application Notes: FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: BAD Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S124 of human BAD.
• Conjugate: PE
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of BAD Antibody (Phospho S124) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)

Related Product Information for anti-BAD antibody

The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq].

Product Categories/Family for anti-BAD antibody

Antibodies; Apoptosis

References

Chen, B., et al. Am. J. Physiol., Cell Physiol. 299 (5), C968-C976 (2010): Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Galmiche, A., et al. Mol. Cancer Res. 8(8):1116-1125(2010). Cerioni, L., et al. Methods Mol. Biol. 648, 291-301 (2010): Yu, B., et al. J. Exp. Clin. Cancer Res. 29, 107 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 10835069
• NCBI GeneID: 572
• NCBI Accession #: NP_004313.1
• NCBI GenBank Nucleotide #: NM_004322.3
• UniProt Accession #: Q92934; O14803; Q6FH21
• Molecular Weight: 18,392 Da
• NCBI Official Full Name: bcl2 antagonist of cell death
• NCBI Official Synonym Full Names: BCL2-associated agonist of cell death
• NCBI Official Symbol: BAD
• NCBI Official Synonym Symbols: BBC2; BCL2L8
• NCBI Protein Information: bcl2 antagonist of cell death; bcl2-L-8; BCL2-binding protein; bcl-2-like protein 8; BCL2-binding component 6; bcl-2-binding component 6; BCL-X/BCL-2 binding protein; BCL2-antagonist of cell death protein; bcl-XL/Bcl-2-associated death promoter
• UniProt Protein Name: Bcl2 antagonist of cell death
• Protein Family: Bcl2-associated agonist of cell death
• UniProt Gene Name: BAD
• UniProt Synonym Gene Names: BBC6; BCL2L8; BAD; Bcl2-L-8
• UniProt Entry Name: BAD_HUMAN

NCBI Description

The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq, Jul 2008]

Uniprot Description

BAD: a proapoptotic member of the Bcl-2 family. Displaces Bax from binding to Bcl-2 and Bcl-xL, resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad inducing the phosphorylation of Bad by Akt and p90RSK. 14-3-3 proteins bind phosphorylated Bad, inhibiting its binding to Bcl-2 and Bcl-xL. Phosphorylation by mitochondria-anchored PKA in the BH3 domain can block the dimerization of Bad and Bcl-xL.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol

Molecular Function: protein kinase B binding; protein binding; protein heterodimerization activity; phospholipid binding; caspase activator activity; protein kinase binding; lipid binding; protein phosphatase 2B binding

Biological Process: response to oleate; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; positive regulation of proteolysis; apoptosis; response to glucocorticoid stimulus; positive regulation of caspase activity; cellular process regulating host cell cycle in response to virus; glucose homeostasis; response to estradiol stimulus; positive regulation of apoptosis by virus; response to glucose stimulus; pore complex biogenesis; positive regulation of autophagy; positive regulation of glucokinase activity; response to drug; caspase activation; epidermal growth factor receptor signaling pathway; release of cytochrome c from mitochondria; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; cytokine and chemokine mediated signaling pathway; suppression by virus of host apoptosis; ADP metabolic process; positive regulation of insulin secretion; response to testosterone stimulus; response to amino acid stimulus; ATP metabolic process; glucose catabolic process; response to ethanol; induction of apoptosis via death domain receptors; DNA damage response, signal transduction resulting in induction of apoptosis; response to hydrogen peroxide; positive regulation of T cell differentiation; positive regulation of B cell differentiation; innate immune response; regulation of mitochondrial membrane permeability; response to calcium ion; response to progesterone stimulus; positive regulation of epithelial cell proliferation