Highly validated and characterized monoclonal/polyclonal
antibodies and recombinant
proteins
The majority of AAA Biotech’s antibodies are highly validated and can be use in multiple
applications such as ELISA, FC,
ICC, IF, IHC, IP, WB, etc. We have antibodies available for rare species, in multiple conjugated
forms or recombinant
antibodies.
As for our high quality proteins, the majority have 90% purity, detected by SDS-PAGE while some are
available in
different tags such as Flag, GST, His, MBP, etc. We also carry high quality native and biologically
active proteins.
AAA Biotech is constantly working to expand our capacity to provide recombinant proteins and
antibodies to most
target proteins.
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LEFT OUTER JOIN `products_extra` as `pe` ON `p`.`products_id` = `pe`.`products_id`
WHERE `p`.`products_id` = '27032'
AND `pd`.`language_id` = 1
LIMIT 1
Query
Database
5.53 ms
select p.*, pd.*,
ifnull(pdns.ncbi_summary, 'N/A') as ncbi_summary_pdns,
ifnull(pdns.sp_comments, 'N/A') as sp_comments_pdns,
ifnull(pdns.ncbi_research_articles, 'N/A') as ncbi_research_articles_pdns,
ifnull(pe.products_description_extra, 'N/A') as products_description_extra
from products p
LEFT OUTER JOIN products_description pd on p.products_id = pd.products_id
LEFT OUTER JOIN products_description_ncbi_sp pdns on p.products_id = pdns.products_id
LEFT OUTER JOIN products_extra pe on p.products_id = pe.products_id
where p.products_id = '27032' and pd.language_id = 1
Query
Database
1.52 ms
SELECT `options_values_price` as `price`, `products_options_values_name` as `package`
FROM `products_attributes`
JOIN `products_options_values` ON `products_options_values`.`products_options_values_id` = `products_attributes`.`options_values_id`
WHERE `products_attributes`.`products_id` = '27032'
Database (4 total Queries, 4 of them unique across 2 Connections)
Time
Query String
2.42 ms
SELECT `p`.*, `pd`.*, IFNULL(pdns.ncbi_summary, "N/A") as ncbi_summary_pdns, IFNULL(pdns.sp_comments, "N/A") as sp_comments_pdns, IFNULL(pdns.ncbi_research_articles, "N/A") as ncbi_research_articles_pdns, IFNULL(pe.products_description_extra, "N/A") as products_description_extra
FROM (`products`, `products` as `p`)
LEFT OUTER JOIN `products_description` as `pd` ON `p`.`products_id` = `pd`.`products_id`
LEFT OUTER JOIN `products_description_ncbi_sp` as `pdns` ON `p`.`products_id` = `pdns`.`products_id`
LEFT OUTER JOIN `products_extra` as `pe` ON `p`.`products_id` = `pe`.`products_id`
WHERE `p`.`products_id` = '27032'
AND `pd`.`language_id` = 1
LIMIT 1
select p.*, pd.*,
ifnull(pdns.ncbi_summary, 'N/A') as ncbi_summary_pdns,
ifnull(pdns.sp_comments, 'N/A') as sp_comments_pdns,
ifnull(pdns.ncbi_research_articles, 'N/A') as ncbi_research_articles_pdns,
ifnull(pe.products_description_extra, 'N/A') as products_description_extra
from products p
LEFT OUTER JOIN products_description pd on p.products_id = pd.products_id
LEFT OUTER JOIN products_description_ncbi_sp pdns on p.products_id = pdns.products_id
LEFT OUTER JOIN products_extra pe on p.products_id = pe.products_id
where p.products_id = '27032' and pd.language_id = 1
SELECT `options_values_price` as `price`, `products_options_values_name` as `package`
FROM `products_attributes`
JOIN `products_options_values` ON `products_options_values`.`products_options_values_id` = `products_attributes`.`options_values_id`
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⇄purity => string (57) "Greater or equal to 85% purity as determined by SDS-PAGE."
$value['purity']
⇄⧉form => string (80) "Lyophilized or liquid (Format to be determined during the manufacturing proc...
$value['form']
Lyophilized or liquid (Format to be determined during the manufacturing process)
⇄concentration => string (3) "N/A"
$value['concentration']
⇄⧉storage_stability => string (195) "Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80...
$value['storage_stability']
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
WB (Western Blot)||Positive WB detected in: His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E. Coli SARS-CoV-2 nucleocapsid antibody at 1:1000; Secondary; Peroxidase-Affinipure Goat Anti-Human IgG, Fc? Fragment Specific at 1/20000 dilution; Predicted band size: 48 kDa; Observed band size: 55 kDa||AAA27032_WB3.jpg!!Biological Activity||Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 5 ug/ml can bind SARS-CoV-2-N Antibody, the EC50 of SARS-CoV-2-N protein is 43.50-118.4 ng/ml.||AAA27032_ACTIVITY2.jpg!!SDS-PAGE||(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.||AAA27032_SDS_PAGE.jpg
⇄⧉etc_term1 => string (136) "Species||Human Novel Coronavirus (2019-nCoV)!!Buffer||Lyophilized from a 0.2...
$value['etc_term1']
Species||Human Novel Coronavirus (2019-nCoV)!!Buffer||Lyophilized from a 0.2 ?m filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.
⇄purity => string (57) "Greater or equal to 85% purity as determined by SDS-PAGE."
$value->a['purity']
⇄⧉form => string (80) "Lyophilized or liquid (Format to be determined during the manufacturing proc...
$value->a['form']
Lyophilized or liquid (Format to be determined during the manufacturing process)
⇄concentration => string (3) "N/A"
$value->a['concentration']
⇄⧉storage_stability => string (195) "Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80...
$value->a['storage_stability']
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
WB (Western Blot)||Positive WB detected in: His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E. Coli SARS-CoV-2 nucleocapsid antibody at 1:1000; Secondary; Peroxidase-Affinipure Goat Anti-Human IgG, Fc? Fragment Specific at 1/20000 dilution; Predicted band size: 48 kDa; Observed band size: 55 kDa||AAA27032_WB3.jpg!!Biological Activity||Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 5 ug/ml can bind SARS-CoV-2-N Antibody, the EC50 of SARS-CoV-2-N protein is 43.50-118.4 ng/ml.||AAA27032_ACTIVITY2.jpg!!SDS-PAGE||(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.||AAA27032_SDS_PAGE.jpg
⇄⧉etc_term1 => string (136) "Species||Human Novel Coronavirus (2019-nCoV)!!Buffer||Lyophilized from a 0.2...
$value->a['etc_term1']
Species||Human Novel Coronavirus (2019-nCoV)!!Buffer||Lyophilized from a 0.2 ?m filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.
⇄purity => string (57) "Greater or equal to 85% purity as determined by SDS-PAGE."
$value->d['purity']
⇄⧉form => string (80) "Lyophilized or liquid (Format to be determined during the manufacturing proc...
$value->d['form']
Lyophilized or liquid (Format to be determined during the manufacturing process)
⇄concentration => string (3) "N/A"
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⇄⧉storage_stability => string (195) "Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80...
$value->d['storage_stability']
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
WB (Western Blot)||Positive WB detected in: His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E. Coli SARS-CoV-2 nucleocapsid antibody at 1:1000; Secondary; Peroxidase-Affinipure Goat Anti-Human IgG, Fc? Fragment Specific at 1/20000 dilution; Predicted band size: 48 kDa; Observed band size: 55 kDa||AAA27032_WB3.jpg!!Biological Activity||Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 5 ug/ml can bind SARS-CoV-2-N Antibody, the EC50 of SARS-CoV-2-N protein is 43.50-118.4 ng/ml.||AAA27032_ACTIVITY2.jpg!!SDS-PAGE||(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.||AAA27032_SDS_PAGE.jpg
⇄⧉etc_term1 => string (136) "Species||Human Novel Coronavirus (2019-nCoV)!!Buffer||Lyophilized from a 0.2...
$value->d['etc_term1']
Species||Human Novel Coronavirus (2019-nCoV)!!Buffer||Lyophilized from a 0.2 ?m filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.
⇄⧉products_description => string (823) "Principle of the Assay: As mentioned above, this kit utilizes the Double Ant...
$value[0]['_source']['products_description']
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Rat MBP monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
⇄products_references => string (3) "N/A"
$value[0]['_source']['products_references']
⇄⧉products_related_diseases => string (261) "Nervous System Diseases||2534!!Demyelinating Diseases||1890!!Brain Diseases|...
$value[0]['_source']['products_related_diseases']
Nervous System Diseases||2534!!Demyelinating Diseases||1890!!Brain Diseases||1631!!Disease Models, Animal||1311!!Encephalomyelitis, Autoimmune, Experimental||1155!!Cardiovascular Diseases||640!!Inflammation||440!!Neoplasms||414!!Hypertension||389!!Necrosis||244
⇄⧉search_terms => string (439) "aaa12462 rat typical testing data standard curve for reference only aaa12462...
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aaa12462 rat typical testing data standard curve for reference only aaa12462_sc elisa kit myelin basic protein mbp golli a1 membrane encephalitogenic 17,343 da mbp_human 1162922 aac41944.1 p02686 q15337 q15338 q15339 q15340 a4fu54 a6ni84 a8my86 a8myl4 b3ky66 b7zks2 b7zks4 159430 samples serum plasma or cell culture supernatant assay type quantitative sandwich detection range 10 ng ml 0.156 sensitivity up to 0.05 intra precision range10
⇄⧉specificity => string (197) "This assay has high sensitivity and excellent specificity for detection of h...
$value[1]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of human MBP antibody. No significant cross-reactivity or interference between human MBP antibody and analogues was observed.
⇄purity => string (3) "N/A"
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⇄form => string (3) "N/A"
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⇄concentration => string (3) "N/A"
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⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
$value[1]['_source']['storage_stability']
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
⇄⧉etc_term2 => string (327) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
$value[1]['_source']['etc_term2']
Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
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⇄products_weight => string (4) "5.00"
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⇄products_status => boolean true
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⇄manufacturers_id => string (3) "700"
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⇄products_name => string (35) "myelin basic protein (MBP) antibody"
Human myelin basic protein antibody; MBP antibody ELISA Kit; myelin basic protein antibody; MBP antibody
⇄products_gene_name => string (3) "N/A"
$value[1]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[1]['_source']['products_gene_name_syn']
⇄⧉products_description => string (581) "Principle of the Assay: This assay employs the quantitative sandwich enzyme ...
$value[1]['_source']['products_description']
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with MBP. Standards and samples are pipetted into the wells with a biotin-conjugated MBP and avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MBP antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.
⇄products_references => string (3) "N/A"
$value[1]['_source']['products_references']
⇄⧉products_related_diseases => string (266) "Nervous System Diseases||2639!!Demyelinating Diseases||1950!!Brain Diseases|...
⇄⧉search_terms => string (577) "aaa15357 human this assay has high sensitivity and excellent specificity for...
$value[1]['_source']['search_terms']
aaa15357 human this assay has high sensitivity and excellent specificity for detection of mbp antibody no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa15357_td elisa kit myelin basic protein partial golli a1 membrane encephalitogenic 17,343 da mbp_human 49456793 cag46717.1 p02686 q15337 q15338 q15339 q15340 a4fu54 a6ni84 a8my86 a8myl4 b3ky66 b7zks2 b7zks4 159430 samples serum cerebrospinal fluid csf type quantitative sandwich range 0.625 ng ml 40 < 0.156 intra precision within an cv ml40
⇄⧉specificity => string (373) "This assay has high sensitivity and excellent specificity for detection of M...
$value[2]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of MBP. No significant cross-reactivity or interference between MBP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MBP and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
$value[2]['_source']['purity']
⇄form => string (3) "N/A"
$value[2]['_source']['form']
⇄concentration => string (3) "N/A"
$value[2]['_source']['concentration']
⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
⇄products_name_oem => string (36) "Mouse Myelin Basic Protein ELISA Kit"
$value[2]['_source']['products_name_oem']
⇄products_name_syn => string (3) "N/A"
$value[2]['_source']['products_name_syn']
⇄products_gene_name => string (3) "MBP"
$value[2]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[2]['_source']['products_gene_name_syn']
⇄⧉products_description => string (1376) "Principle of the Assay: MBP ELISA kit applies the competitive enzyme immunoa...
$value[2]['_source']['products_description']
Principle of the Assay: MBP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MBP antibody and an MBP-HRP conjugate. The assay sample and buffer are incubated together with MBP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MBP concentration since MBP from samples and MBP-HRP conjugate compete for the anti-MBP antibody binding site. Since the number of sites is limited, as more sites are occupied by MBP from the sample, fewer sites are left to bind MBP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MBP concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This MBP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MBP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄products_references => string (3) "N/A"
$value[2]['_source']['products_references']
⇄⧉products_related_diseases => string (266) "Nervous System Diseases||2646!!Demyelinating Diseases||1954!!Brain Diseases|...
⇄⧉search_terms => string (714) "aaa16366 mouse this assay has high sensitivity and excellent specificity for...
$value[2]['_source']['search_terms']
aaa16366 mouse this assay has high sensitivity and excellent specificity for detection of mbp no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa16366_sc elisa kit myelin basic protein partial golli a1 membrane encephalitogenic 17,343 da mbp_human 49456793 cag46717.1 p02686 q15337 q15338 q15339 q15340 a4fu54 a6ni84 a8my86 a8myl4 b3ky66 b7zks2 b7zks4 159430 neurobiology samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive 1.0 pg ml competitive1.0
⇄⧉specificity => string (373) "This assay has high sensitivity and excellent specificity for detection of M...
$value[3]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of MBP. No significant cross-reactivity or interference between MBP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MBP and all the analogues, therefore, cross reaction may still exist in some cases.
⇄purity => string (3) "N/A"
$value[3]['_source']['purity']
⇄form => string (3) "N/A"
$value[3]['_source']['form']
⇄concentration => string (3) "N/A"
$value[3]['_source']['concentration']
⇄storage_stability => string (35) "Store all reagents at 2-8 degree C."
Assay Type||Quantitative Competitive or Sandwich!!Samples||Serum, plasma, cell culture supernatants, body fluid and tissue homogenate!!Sensitivity||1.0 pg/mL
⇄products_name_oem => string (34) "Rat Myelin Basic Protein ELISA Kit"
$value[3]['_source']['products_name_oem']
⇄products_name_syn => string (3) "N/A"
$value[3]['_source']['products_name_syn']
⇄products_gene_name => string (3) "MBP"
$value[3]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[3]['_source']['products_gene_name_syn']
⇄⧉products_description => string (1491) "Principle of the Assay: MBP ELISA kit applies the quantitative sandwich enzy...
$value[3]['_source']['products_description']
Principle of the Assay: MBP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for MBP. Standards or samples are then added to the microtiter plate wells and MBP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MBP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for MBP are added to each well to "sandwich" the MBP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MBP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MBP concentration in each sample is interpolated from this standard curve.<br><br>Intended Uses: This MBP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat MBP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
⇄products_references => string (3) "N/A"
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⇄⧉products_related_diseases => string (266) "Nervous System Diseases||2646!!Demyelinating Diseases||1954!!Brain Diseases|...
⇄⧉search_terms => string (705) "aaa16405 rat this assay has high sensitivity and excellent specificity for d...
$value[3]['_source']['search_terms']
aaa16405 rat this assay has high sensitivity and excellent specificity for detection of mbp no significant cross reactivity or interference between analogues was observed note limited by current skills knowledge it is impossible us to complete the all therefore reaction may still exist in some cases typical testing data standard curve reference only aaa16405_sc elisa kit myelin basic protein partial golli a1 membrane encephalitogenic 17,343 da mbp_human 49456793 cag46717.1 p02686 q15337 q15338 q15339 q15340 a4fu54 a6ni84 a8my86 a8myl4 b3ky66 b7zks2 b7zks4 159430 neurobiology samples serum plasma cell culture supernatants body fluid tissue homogenate type quantitative competitive sandwich 1.0pg ml
⇄⧉specificity => string (179) "This assay has high sensitivity and excellent specificity for detection of h...
$value[4]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of human MBP. No significant cross-reactivity or interference between human MBP and analogues was observed.
⇄purity => string (3) "N/A"
$value[4]['_source']['purity']
⇄form => string (3) "N/A"
$value[4]['_source']['form']
⇄concentration => string (3) "N/A"
$value[4]['_source']['concentration']
⇄⧉storage_stability => string (129) "Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please ...
$value[4]['_source']['storage_stability']
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
⇄⧉etc_term2 => string (327) "Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV...
$value[4]['_source']['etc_term2']
Intra-assay Precision||Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.!!Inter-assay Precision||Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Human myelin basic protein; MBP ELISA Kit; MGC99675; Golli-mbp; OTTHUMP00000174383; myelin basic protein
⇄products_gene_name => string (3) "MBP"
$value[4]['_source']['products_gene_name']
⇄products_gene_name_syn => string (3) "N/A"
$value[4]['_source']['products_gene_name_syn']
⇄⧉products_description => string (727) "Principle of the Assay: This assay employs the quantitative sandwich enzyme ...
$value[4]['_source']['products_description']
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MBP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MBP bound in the initial step. The color development is stopped and the intensity of the color is measured.
⇄products_references => string (3) "N/A"
$value[4]['_source']['products_references']
⇄⧉products_related_diseases => string (266) "Nervous System Diseases||2639!!Demyelinating Diseases||1950!!Brain Diseases|...
⇄⧉search_terms => string (652) "aaa14968 human this assay has high sensitivity and excellent specificity for...
$value[4]['_source']['search_terms']
aaa14968 human this assay has high sensitivity and excellent specificity for detection of mbp no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa14968_td elisa kit myelin basic protein mgc99675 golli otthump00000174383 isoform 1 a1 membrane encephalitogenic 33,117 da mbp_human 68509930 np_001020252.1 p02686 nm_001025081.1 q15337 q15338 q15339 q15340 a4fu54 a6ni84 a8my86 a8myl4 b3ky66 b7zks2 b7zks4 159430 samples serum plasma tissue homogenates cell culture supernates type quantitative sandwich range 0.156 ng ml 10 < 0.039 intra precision within an cv isoform1 ml10
⇄⧉specificity => string (167) "This assay has high sensitivity and excellent specificity for detection of M...
$value[5]['_source']['specificity']
This assay has high sensitivity and excellent specificity for detection of MBP. No significant cross-reactivity or interference between MBP and analogues was observed.
⇄purity => string (3) "N/A"
$value[5]['_source']['purity']
⇄form => string (3) "N/A"
$value[5]['_source']['form']
⇄concentration => string (3) "N/A"
$value[5]['_source']['concentration']
⇄⧉storage_stability => string (156) "Store entire kit at 2-8C for short-term. For longer-term, please store the m...
$value[5]['_source']['storage_stability']
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
MBP/Hmbpr/mld/shi/MGC99675/Myelin A1 protein/myelin basic protein/Myelin membrane encephalitogenic protein
⇄products_gene_name => string (3) "MBP"
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Nervous System Diseases||2772!!Demyelinating Diseases||2002!!Brain Diseases||1763!!Encephalomyelitis, Autoimmune, Experimental||1208!!Cardiovascular Diseases||726!!Inflammation||520!!Hypertension||424!!Necrosis||271!!Heart Diseases||204!!Kidney Diseases||153
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aaa17604 mouse this assay has high sensitivity and excellent specificity for detection of mbp no significant cross reactivity or interference between analogues was observed typical testing data standard curve reference only aaa17604_sc elisa kit myelin basic protein hmbpr mld shi mgc99675 a1 membrane encephalitogenic golli 17,343 da mbp_human 1162922 aac41944.1 p02686 q15337 q15338 q15339 q15340 a4fu54 a6ni84 a8my86 a8myl4 b3ky66 b7zks2 b7zks4 159430 samples serum plasma tissue homogenates other biological fluids type quantitative sandwich range 0.156 10ng ml 0.094ng intra precision cv
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Store at -20 degree C. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freeze-thaw cycles.
WB: Concentration 0.1-0.5ug/ml; Tested Species: Ms, Rat<br>IHC (Paraffin-embedded Section): Concentration: 2-5ug/ml; Tested Species: Ms, Rat; Antigen Retrieval: By Heat<br>IF: Concentration: 2ug/ml; Tested Species: Ms, Rat<br><br>Tested Species: In-house tested species with positive results.<br>Predicted Species: Species predicted to be fit for the product based on sequence similiarities.<br>By Heat: Boiling paraffin sections in 10mM citrate buffer pH 6.0, for 20 minutes is required for the staining of formalin/paraffin sections.<br><br>Other applications have not been tested. Optimal dilutions should be determined by end users.
IHC (Immunohistochemistry)||Anti-Myelin Basic Protein antibody, AAA11509, IHC(P)<br>IHC(P): Rat Brain Tissue||AAA11509_IHC2.jpg!!WB (Western Blot)||Anti-Myelin Basic Protein antibody, AAA11509, Western blotting<br>WB: Mouse Brain Tissue Lysate||AAA11509_WB.jpg
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Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Myelin Basic Protein (182-197aa KLGGRDSRSGSPMARR), identical to the related rat and mouse sequences.
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Reconstitution||0.2ml of distilled water will yield a concentration of 500ug/ml.
<b>Description: </b>Rabbit IgG polyclonal antibody for Myelin basic protein(MBP) detection. Tested with WB, IHC-P in Human, Mouse, Rat.<br><b>Background: </b>Myelin basic protein(MBP) is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the central nervous system and the peripheral nervous system, respectively. It is most abundant in hemopoietic system and contains seven exons distributed over 32-34 kb. MBP isolated from MS brain may differ in charge microheterogeneity which would affect antigenic determinants. MBP is mapped to chromosome 18q22-23. Failure in this gene expression would be correlated in the central white matter with extrapyramidal system degeneration signs. Moreover, it is a candidate autoantigen in the disease multiple sclerosis.
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⇄⧉search_terms => string (1260) "aaa11509 rabbit human mouse rat polyclonal igg immunogen affinity purified l...
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aaa11509 rabbit human mouse rat polyclonal igg immunogen affinity purified lyophilized western blot wb immunohistochemistry ihc paraffin immunofluoresence if concentration 0.1 0.5ug ml tested species hu ms embedded section 0.5 1ug predicted antigen retrieval by heat 2ug in house with positive results to be fit for the product based on sequence similiarities boiling sections 10mm citrate buffer ph 6.0 20 minutes is required staining of formalin other applications have not been optimal dilutions should determined end users antibody can supporter chemiluminescence kit mbs176460 supported mbs176451 p anti myelin basic protein blotting brain tissue lysate aaa11509_wb aaa11509_ihc2 mbp gdb golli hemopoietic hmbpr hugo mbp_human mgc99675 mld a1 deficient membrane encephalitogenic otthump00000163776 otthump00000174387 otthump00000174388 shi shiverer sp 17378805 p02686.3 p02686 q15337 q15338 q15339 q15340 a4fu54 a6ni84 a8my86 a8myl4 b3ky66 b7zks2 b7zks4 159430 a synthetic peptide corresponding at c terminus 182 197aa klggrdsrsgspmarr identical related and sequences contents each vial contains 5mg bsa 0.9mg nacl 0.2mg na2hpo4 0.05mg thimerosal nan3 reconstitution add 0.2ml distilled water will yield 500ug concentration0.1 section0.5 ph6.0 terminus182
⇄⧉specificity => string (334) "Myelin Basic Protein/MBP Antibody detects endogenous levels of total Myelin ...
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Myelin Basic Protein/MBP Antibody detects endogenous levels of total Myelin Basic Protein/MBP.<br>Tissue Specificity: MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
⇄⧉purity => string (92) "The antiserum was purified by peptide affinity chromatography using SulfoLin...
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The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
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Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
⇄⧉testing_protocols => string (1996) "IHC (Immunohistchemistry)||At 1/100 staining Rat brain tissue by IHC-P. The ...
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IHC (Immunohistchemistry)||At 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.||AAA31339_IHC6.jpg!!IHC (Immunohistochemistry)||At 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.||AAA31339_IHC5.jpg!!IHC (Immunohistochemistry)||At 1/100 staining Mouse thymus tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.||AAA31339_IHC4.jpg!!IHC (Immunohistochemistry)||At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.||AAA31339_IHC3.jpg!!IHC (Immunohistochemistry)||Staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.||AAA31339_IHC2.jpg!!WB (Western Blot)||Western blot analysis of extract from Rat brain, Mouse brain and Rat hippocampus Tissue using MBP Antibody||AAA31339_WB.jpg
⇄⧉etc_term1 => string (189) "Immunogen||A synthesized peptide derived from human MBP (myelin basic protei...
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Immunogen||A synthesized peptide derived from human MBP (myelin basic protein), corresponding to a region within the internal amino acids.!!Conjugation||Unconjugated!!Fragment||Fab fragment
⇄⧉etc_term2 => string (1158) "Post Translational Modifications||Several charge isomers of MBP; C1 (the mos...
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Post Translational Modifications||Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A (H), C8-A (L), C8-B (H) and C8-B (L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6). Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1.!!Subunit Structure||Homodimer. Isoform 3 exists as a homodimer.!!Similarity||Belongs to the myelin basic protein family.!!Subcellular Location||Myelin membrane>Peripheral membrane protein>Cytoplasmic side.<br>Note: Cytoplasmic side of myelin.Nucleus.<br>Note: Targeted to nucleus in oligodendrocytes.
⇄⧉products_description => string (1600) "The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the mo...
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The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.<br>The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
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aaa31339 rabbit human mouse rat predicted reactivity pig 100 bovine horse dog chicken 86 xenopus polyclonal supplied at 1.0mg ml in phosphate buffered saline without mg2+ and ca2+ ph7.4 150mm nacl 0.02 sodium azide 50 glycerol the antiserum was purified by peptide affinity chromatography using sulfolink coupling resin liquid myelin basic protein mbp antibody detects endogenous levels of total tissue specificity isoforms are found both central peripheral nervous system whereas golli expressed fetal thymus spleen spinal cord as well cell lines derived from immune western blot wb immunohistochemistry ihc immunofluorescence if immunocytochemistry icc elisa eia 1:500 1:1000 1:50 1:200 1:100 1:20,000 1:40,000 analysis extract brain hippocampus aaa31339_wb staining p sample formaldehyde fixed a heat mediated antigen retrieval step citrate buffer performed then blocked incubated with primary 4 degree c overnight an hrp conjugated anti used secondary aaa31339_ihc2 1 aaa31339_ihc3 aaa31339_ihc4 aaa31339_ihc5 aaa31339_ihc6 gdb hemopoietic hmbpr hugo mbp_cavpo mbp_human mgc99675 mld a1 deficient membrane encephalitogenic otthump00000163776 otthump00000174387 otthump00000174388 shi shiverer sp 33,117 da 1162922 aac41944.1 p02686 q15337 q15338 q15339 q15340 a4fu54 a6ni84 a8my86 a8myl4 b3ky66 b7zks2 b7zks4 159430 immunogen synthesized corresponding to region within internal amino acids conjugation unconjugated fragment fab post translational modifications several charge isomers c1 most cationic least modified abundant form c2 c3 c4 c5 c6 c7 c8 b produced result optional ptm such phosphorylation deamidation glutamine or asparagine arginine citrullination methylation contain each two mass termed h l standing for higher lower molecular weight phosphorylated ratio methylated residues decreases during aging making more cationic.the n terminal alanine is acetylated isoform 3 5 6 arg 241 be monomethylated 60 symmetrically dimethylated.phosphorylated taok2 vrk2 mapk11 mapk12 mapk14 mink1 subunit structure homodimer exists similarity belongs family subcellular location membrane>peripheral protein>cytoplasmic side note cytoplasmic myelin.nucleus targeted nucleus oligodendrocytes pig100 chicken86 azide50 primary4 aaa31339_ihc21 isoform3 arg241 monomethylated60
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Intra-assay Precision||Intra-assay CV (%) are less than 15%.!!Inter-assay Precision||Inter-assay CV (?) are less than 15%.
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Intended Uses: The kit uses a sandwich enzyme-linked immunosorbent assay (ELISA) to qualitatively analyze Human Hepatitis D Virus Antigen (HDVAg) in Human serum, plasma. This kit is in vitro research use only! Not for therapeutic or test applications!<br><br>Principle of the Assay: The kit uses a sandwich enzyme-linked immunosorbent assay (ELISA) to qualitatively analyze Human Hepatitis D Virus Antigen (HDVAg) in Human serum, plasma. This kit is in vitro research use only! Not for therapeutic or test applications!<br><br>Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used for determine the level of HBVCAg (hereafter termed "analyte") in undiluted original Human serum, plasma or tissue homogenates samples. For other sample types please contact tech support to determine compatibility with this assay. This kit is not suitable for assaying non-biological sources of substances.
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Background: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or diagnostic applications! This kit is intended to be used for determine the level of IgLL1 (hereafter termed "analyte") in undiluted original Pigeon serum, plasma or tissue homogenates samples. For other sample types please contact tech support to determine compatibility with this assay. This kit is not suitable for assaying non-biological sources of substances.<br><br>Principle of the Assay: The kit uses a sandwich enzyme-linked immunosorbent assay (ELISA) to qualitatively analyze Human Coronavirus HKU- 39849 Nucleocapsid Antibody (Anti-HCoV-HKU-NP) in Human serum, plasma. This kit is in vitro research use only! Not for therapeutic or diagnostic applications!<br><br>Intended Uses: The kit uses a sandwich enzyme-linked immunosorbent assay (ELISA) to qualitatively analyze Human Coronavirus HKU- 39849 Nucleocapsid Antibody (Anti-HCoV-HKU-NP) in Human serum, plasma. This kit is in vitro research use only! Not for therapeutic or diagnostic applications!
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Samples||Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids!!Assay Type||Sandwich!!Detection Range||25 ng/ml - 200 ng/ml.!!Sensitivity||1.0 ng/ml.
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Intended Uses||ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample. This quantitative Sandwich ELISA kit is intended to determinate ADAMTS4 concentrations in Rabbit serum, plasma, tissue homogenates, feces, urine and body fluids, and it is only for research, not for drug, household, therapeutic applications!!Intra-assay Precision||Intra-assay CV (%) are less than 15%.!!Inter-assay Precision||Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
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Rabbit A Disintegrin Like and Metalloproteinase With Thrombospondin Type 1 Motif 4 ELISA Kit
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⇄⧉products_description => string (784) "<b>Introduction: </b>ELISA is a simple and highly sensitive method of analys...
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<b>Introduction: </b>ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample. This quantitative Sandwich ELISA kit is intended to determinate ADAMTS4 concentrations in Rabbit serum, plasma, tissue homogenates, feces, urine and body fluids, and it is only for research!
⇄⧉ncbi_protein_info => string (194) "A disintegrin and metalloproteinase with thrombospondin motifs 4; ADAM-TS4; ...
$value[10]['_source']['ncbi_protein_info']
A disintegrin and metalloproteinase with thrombospondin motifs 4; ADAM-TS4; ADAM-TS 4; aggrecanase-1; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4
⇄⧉search_terms => string (1214) "aaa10094 rabbit no significant cross reactivity or interference between adam...
$value[10]['_source']['search_terms']
aaa10094 rabbit no significant cross reactivity or interference between adamts4 and analogues was observed typical testing data standard curve for reference only aaa10094_td elisa kit a disintegrin like metalloproteinase with thrombospondin type 1 motif 4 motifs preproprotein adam metallopeptidase admp adamts 2 ts4 ts aggrecanase metalloprotease reprolysin 90,197 da kiaa0688 ats4_human 157427675 np_005090.3 o75173 nm_005099.4 q5vtw2 q6p4q8 q6uwa8 q9un83 603876 samples serum plasma tissue homogenate feces urine body fluids assay sandwich detection range 25 ng ml 200 sensitivity 1.0 intended uses is simple highly sensitive method of analysis that allows simultaneous rapid quantification large number the based on specific recognition target compound analyte antigen by antibodies which bind to antibody complex detected measured aid an enzyme labeled upon addition non colored reagent produces color reaction where intensity directly inversely proportional concentration in sample this quantitative determinate concentrations homogenates it research not drug household therapeutic applications intra precision cv are less than 15 inter = sd mean x100 type1 motif4 adamts2 range25 ml200 sensitivity1.0 than15
⇄⧉products_description => string (1104) "Intended Uses: This immunoassay kit allows for the in vitro quantitative det...
$value[11]['_source']['products_description']
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.<br><br>Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
⇄products_references => string (3) "N/A"
$value[11]['_source']['products_references']
⇄⧉products_related_diseases => string (268) "Nervous System Diseases||257!!Brain Diseases||212!!Pelizaeus-Merzbacher Dise...
⇄⧉search_terms => string (315) "aaa23143 rat typical testing data standard curve for reference only aaa23143...
$value[11]['_source']['search_terms']
aaa23143 rat typical testing data standard curve for reference only aaa23143_sc elisa kit myelin proteolipid protein plp lipophilin plp1 1 30,077 da mypr_rat 13591880 np_112252.1 p60203 nm_030990.2 p04400 p06905 q561k5 samples serum plasma tissue homogenates cell culture supernates or other biological fluids plp11
