<
Western Blot (WB)
(Western Blot: Sample: Recombinant BPI, Mouse.)
Rabbit anti-Mouse Bactericidal/Permeability Increasing Protein Polyclonal Antibody | anti-BPI antibody
Polyclonal Antibody to Bactericidal/Permeability Increasing Protein (BPI)
Gene Names
Bpi; Bpifd1; 9230105K17Rik
Reactivity
Mouse
Applications
ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen-specific Affinity Chromatography.
Synonyms
Bactericidal/Permeability Increasing Protein; Polyclonal Antibody; Polyclonal Antibody to Bactericidal/Permeability Increasing Protein (BPI); anti-BPI antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against BPI. It has beenselected for its ability to recognize BPI in immunohistochemical staining andwestern blotting.
Purity/Purification
Antigen-specific Affinity Chromatography.
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence Length
482
Applicable Applications for anti-BPI antibody
WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
Application Notes
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Source
Polyclonal antibody preparation
Traits
Liquid
Cross Reactivity
Mouse
Immunogen
Recombinant BPI (Asn320~Pro457) expressed in E.coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2052929
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4 degre C for frequent use.
Aliquot and store at -20 degre C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degre C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 4 degre C for frequent use.
Aliquot and store at -20 degre C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degre C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Recombinant BPI, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant BPI, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Skeletal Muscle Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Skeletal Muscle Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,352 Da
NCBI Official Full Name
bactericidal permeability-increasing protein isoform 1
NCBI Official Synonym Full Names
bactericidal permeablility increasing protein
NCBI Official Symbol
Bpi
NCBI Official Synonym Symbols
Bpifd1; 9230105K17Rik
NCBI Protein Information
bactericidal permeability-increasing protein
UniProt Protein Name
Bactericidal permeability-increasing protein
Protein Family
UniProt Gene Name
Bpi
UniProt Synonym Gene Names
BPI
Uniprot Description
BPI: The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.
Protein type: Cell surface; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 2|2 H1
Cellular Component: cytoplasm; extracellular region; extracellular space; membrane
Molecular Function: lipid binding; lipopolysaccharide binding
Biological Process: defense response to bacterium; defense response to Gram-negative bacterium; immune response; negative regulation of interleukin-6 production; negative regulation of interleukin-8 production; negative regulation of macrophage activation; negative regulation of tumor necrosis factor production
Research Articles on BPI
Similar Products
Product Notes
The BPI bpi (Catalog #AAA2026443) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Bactericidal/Permeability Increasing Protein (BPI) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Bactericidal/Permeability Increasing Protein can be used in a range of immunoassay formats including, but not limited to, WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM. Western blotting: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the BPI bpi for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bactericidal/Permeability Increasing Protein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
