Rabbit Bacillus cereus Phospholipase C Polyclonal Antibody
Rabbit anti Bacillus cereus Phospholipase C, conjugated with Biotin
Reactivity
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Applications
ELISA, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry, Dot Blot, Immunoblot, Western Blot
Purity
The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography
Synonyms
Bacillus cereus Phospholipase C; Polyclonal Antibody; Rabbit anti Bacillus cereus Phospholipase C; conjugated with Biotin; anti-Bacillus cereus Phospholipase C antibody
Host
Rabbit
Reactivity
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Clonality
Polyclonal
Specificity
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition.
Purity/Purification
The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography
Form/Format
Biotin-coupled hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). 1 vial with 1ml immunoconjugate, lyophilized from PBS
Applicable Applications for anti-Bacillus cereus Phospholipase C antibody
ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), Dot Blot (DB), Immunoblotting (IB)
Application Notes
This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as eg., ELISA and Western Blotting and Immunofluorescence or Histochemical techniques). Direction for use: Working dilution in non-precipitating antibody-binding techniques may vary widely, but may be between 1/1,000 and 1/20,000
Conjugation
Biotin
Preservative
No preservatived added, as it may interfere with the antibody activity. No foreign protein added
Immunogen
Phospholipase C isolated and purified from Bacillus cereus. Freund's complete adjuvant is used in the first step of the immunization procedure.
Physicochemical Characteristics
IgG protein concentration 10mg/ml. Biotin/IgG protein molar ration (B/P) approximately 5.4. No foreign proteins added.
Marker
N-Hydroxysuccinimidobiotin.
Conjugation
A proprietary techniques for the binding to biotin is used, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assur stability and long shelf life.
Preparation and Storage
The lyophilized conjugate is shipped at ambient temperature and may be stored at 4 degree C; prolonged storage at or below -20 degree C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below - 20 degree C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4 degree C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Related Product Information for anti-Bacillus cereus Phospholipase C antibody
Nordenzyme is the name of a group of polyclonal immunologic reagents to enzymes fractions (7S) of the specific antisera and the isolated antibodies (PAb) to the enzymes. Products against some 180 enzymes are available either as 7S or PAb or in both forms. The 7S fractions are also available in biotinylated form. The Nordenzyme products have been obtained by immunization with highly purified enzymes.
Similar Products
Product Notes
The Bacillus cereus Phospholipase C (Catalog #AAA573066) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Bacillus cereus Phospholipase C, conjugated with Biotin reacts with Cross-reactivities against enzymes of other sources may occur but have not been determined. and may cross-react with other species as described in the data sheet. AAA Biotech's Bacillus cereus Phospholipase C can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), Dot Blot (DB), Immunoblotting (IB). This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as eg., ELISA and Western Blotting and Immunofluorescence or Histochemical techniques). Direction for use: Working dilution in non-precipitating antibody-binding techniques may vary widely, but may be between 1/1,000 and 1/20,000. Researchers should empirically determine the suitability of the Bacillus cereus Phospholipase C for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bacillus cereus Phospholipase C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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