Western Blot (WB)
(Western Blot using MBS6004156 at 1:2000 Lane 1: 293T/17 whole cell lysate Lane 2: Daudi whole cell lysate Lane 3: K562 whole cell lysate Lane 4: Ramos whole cell lysate Lysates/proteins at 20ug/lane. Secondary IgG, (H+L) (HRP) Goat Anti-Rabbit Predicted band size : 93kD Blocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human BACH2 Polyclonal Antibody | anti-BACH2 antibody
BACH2, CT (BACH2, Transcription regulator protein BACH2, BTB and CNC homolog 2) (FITC)
Gene Names
BACH2; BTBD25
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Functional Assay
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Synonyms
BACH2; Polyclonal Antibody; CT (BACH2; Transcription regulator protein BACH2; BTB and CNC homolog 2) (FITC); BTB and CNC homolog 2; anti-BACH2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human BACH2.
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-BACH2 antibody
FLISA, Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 815-841 in the C-terminal region of human BACH2.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot using MBS6004156 at 1:2000 Lane 1: 293T/17 whole cell lysate Lane 2: Daudi whole cell lysate Lane 3: K562 whole cell lysate Lane 4: Ramos whole cell lysate Lysates/proteins at 20ug/lane. Secondary IgG, (H+L) (HRP) Goat Anti-Rabbit Predicted band size : 93kD Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Western Blot using MBS6004156 at 1:2000 Lane 1: 293T/17 whole cell lysate Lane 2: Daudi whole cell lysate Lane 3: K562 whole cell lysate Lane 4: Ramos whole cell lysate Lysates/proteins at 20ug/lane. Secondary IgG, (H+L) (HRP) Goat Anti-Rabbit Predicted band size : 93kD Blocking/Dilution buffer: 5% NFDM/TBST.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of MDA-MB435 cells using MBS6004156 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibody was used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of MDA-MB435 cells using MBS6004156 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibody was used for the analysis.)
Testing Data
(Overlay histogram showing Ramos cells stained withMBS6004156. The cells were fixed with 2% paraformaldehyde (10mins) and then permeabilized with 90% methanol for 10mins. The cells were then incubated in 2% BSA to block non-specific protein-protein interactions followed by MBS6004156 (1:25) for 60mins at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1:200 for 40mins at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10e6 cells) used under the same conditions. Acquisition of >10,000 events was performed.)
Testing Data
(Overlay histogram showing Ramos cells stained withMBS6004156. The cells were fixed with 2% paraformaldehyde (10mins) and then permeabilized with 90% methanol for 10mins. The cells were then incubated in 2% BSA to block non-specific protein-protein interactions followed by MBS6004156 (1:25) for 60mins at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1:200 for 40mins at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10e6 cells) used under the same conditions. Acquisition of >10,000 events was performed.)
Product Categories/Family for anti-BACH2 antibody
References
1. Hinks, A., et al. Ann. Rheum. Dis. (2010). In press: 2. Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010). 3. Dubois, P.C., et al. Nat. Genet. 42(4):295-302(2010). 4. Pierce, B.L., et al. Hum. Hered. 69(3):193-201(2010). 5. Barrett, J.C., et al. Nat. Genet. 41(6):703-707(2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
92,537 Da
NCBI Official Full Name
transcription regulator protein BACH2
NCBI Official Synonym Full Names
BTB and CNC homology 1, basic leucine zipper transcription factor 2
NCBI Official Symbol
BACH2
NCBI Official Synonym Symbols
BTBD25
NCBI Protein Information
transcription regulator protein BACH2; BTB and CNC homolog 2
UniProt Protein Name
Transcription regulator protein BACH2
Protein Family
UniProt Gene Name
BACH2
UniProt Entry Name
BACH2_HUMAN
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Product Notes
The BACH2 bach2 (Catalog #AAA6277272) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BACH2, CT (BACH2, Transcription regulator protein BACH2, BTB and CNC homolog 2) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BACH2 can be used in a range of immunoassay formats including, but not limited to, FLISA, Western Blot (WB), Flow Cytometry (FC/FACS). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the BACH2 bach2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BACH2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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