Rabbit anti-Human CBX5 Polyclonal Antibody | anti-CBX5 antibody

CBX5 Antibody

Cat. Num. AAA7004680

• Gene Names: CBX5; HP1; HP1A; HEL25
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Caprylic Acid Ammonium Sulfate Precipitation purified
• Synonyms: CBX5; Polyclonal Antibody; CBX5 Antibody; Chromobox protein homolog 5; Antigen p25; Heterochromatin protein 1 homolog alpha; HP1 alpha; HP1A; anti-CBX5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Caprylic Acid Ammonium Sulfate Precipitation purified
• Form/Format: Liquid
• Sequence Length: 191

• Applicable Applications for anti-CBX5 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: WB:1:500-2000, IHC:1:20-1:200
• Immunogen: Recombinant human Chromobox protein homolog 5 protein
• Conjugation: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-10210 / sc-30742 / sc-30743 / sc-10217 / sc-10215 / sc-28735
• Preparation and Storage: Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western blotAll lanes: CBX5 antibody at 9 ug/mlLane 1: K562 whole cell lysateLane 2: MCF7 whole cell lysateLane 3: 293T whole cell lysateLane 4: Hela whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 22 kDaObserved band size: 22 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human glioma cancer using MBS7004680 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human brain tissue using MBS7004680 at dilution of 1:100)

Related Product Information for anti-CBX5 antibody

Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 188035908
• NCBI GeneID: 23468
• NCBI Accession #: NP_001120793.1
• NCBI GenBank Nucleotide #: NM_001127321.1
• UniProt Accession #: P45973; B2R8T9
• Molecular Weight: 22,225 Da
• NCBI Official Full Name: chromobox protein homolog 5
• NCBI Official Synonym Full Names: chromobox 5
• NCBI Official Symbol: CBX5
• NCBI Official Synonym Symbols: HP1; HP1A; HEL25
• NCBI Protein Information: chromobox protein homolog 5
• UniProt Protein Name: Chromobox protein homolog 5
• Protein Family: Chromobox protein
• UniProt Gene Name: CBX5
• UniProt Synonym Gene Names: HP1A; HP1 alpha
• UniProt Entry Name: CBX5_HUMAN

NCBI Description

This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

HP1 alpha: Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Interacts with SUV420H1 and SUV420H2. Interacts with HP1BP3. Interacts directly with ATRX, CHAF1A, LBR, NIPBL, SP100, STAM2 and TRIM28 via the chromoshadow domain. Can interact directly with CBX3 via the chromoshadow domain. Interacts with histone H3 methylated at 'Lys- 9'. Interacts with BAHD1, MIS12 and DSN1. Interacts with POGZ; POGZ and PXVXL motif-containing proteins such as INCENP and TRIM28 compete for interaction with CBX5. Interacts with INCENP and TRIM24. Interacts with JC virus agnoprotein; this interaction induces the dissociation of CBX5 from LBR, resulting in destabilization of the nuclear envelope. Interacts with CHAMP1. Interacts with ASXL1.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: centric heterochromatin; chromocenter; histone deacetylase complex; histone methyltransferase complex; kinetochore; nuclear chromosome, telomeric region; nuclear envelope; nuclear heterochromatin; nucleolus; nucleoplasm; nucleus; PML body; transcriptional repressor complex

Molecular Function: chromatin binding; histone deacetylase binding; methylated histone residue binding; protein binding; protein binding, bridging; protein homodimerization activity

Biological Process: blood coagulation; negative regulation of transcription, DNA-dependent; viral reproduction