Rabbit anti-Human BACE Polyclonal Antibody | anti-BACE antibody

BACE, Phosphorylated (Ser498) (Beta-secretase 1, Beta-site APP Cleaving Enzyme 1, Beta-site Amyloid Precursor Protein Cleaving Enzyme 1, Aspartyl Protease 2, Asp 2, ASP2, Membrane-associated Aspartic Protease 2, Memapsin-2, BACE1, BACE, KIAA1149) (FITC)

Cat. Num. AAA6464654

• Gene Names: BACE1; ASP2; BACE; HSPC104
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: BACE; Polyclonal Antibody; Phosphorylated (Ser498) (Beta-secretase 1; Beta-site APP Cleaving Enzyme 1; Beta-site Amyloid Precursor Protein Cleaving Enzyme 1; Aspartyl Protease 2; Asp 2; ASP2; Membrane-associated Aspartic Protease 2; Memapsin-2; BACE1; KIAA1149) (FITC); anti-BACE antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human BACE when phosphorylated at Ser498.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-BACE antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser498 of human BACE (KLH).
• Conjugate: FITC
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-Phospho-BACE-S498 Pab is used in Western blot to detect Phospho-BACE-S498 in mouse brain tissue lysate)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-BACE antibody

Antibodies; Apoptosis

References

1. Xie, J., et al., J. Biol. Chem. 280(14):13824-13832 (2005). 2. He, X., et al., J. Biol. Chem. 280(12):11696-11703 (2005). 3. Huang, X.P., et al., J. Biol. Chem. 279(36):37886-37894 (2004). 4. Chiocco, M.J., et al., J. Biol. Chem. 279(50):52535-52542 (2004). 5. Yang, H.C., et al., J. Neurochem. 91(6):1249-1259 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 6912266
• NCBI GeneID: 23621
• NCBI Accession #: NP_036236
• NCBI GenBank Nucleotide #: NM_012104
• UniProt Accession #: P56817; Q9BYB9; Q9BYC0; Q9BYC1; Q9UJT5; Q9ULS1; A0M8W7; B0YIU9; E9PE65; H7BXJ9
• Molecular Weight: 42,107 Da
• NCBI Official Full Name: beta-secretase 1 isoform A preproprotein
• NCBI Official Synonym Full Names: beta-site APP-cleaving enzyme 1
• NCBI Official Symbol: BACE1
• NCBI Official Synonym Symbols: ASP2; BACE; HSPC104
• NCBI Protein Information: beta-secretase 1; APP beta-secretase; asp 2; aspartyl protease 2; beta-secretase 1 precursor variant 1; beta-site APP cleaving enzyme 1; beta-site amyloid beta A4 precursor protein-cleaving enzyme; memapsin-2; membrane-associated aspartic protease 2; tran
• UniProt Protein Name: Beta-secretase 1
• Protein Family: Beta-secretase
• UniProt Gene Name: BACE1
• UniProt Synonym Gene Names: BACE; KIAA1149; ASP2; Asp 2
• UniProt Entry Name: BACE1_HUMAN

Uniprot Description

BACE: an integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Cleaves APP at the amino terminus of the A-beta peptide sequence, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated carboxy-terminal fragment which is later release by gamma-secretase. Four splice-variant isoforms have been described.

Protein type: Protease; EC 3.4.23.46; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23.2-q23.3

Cellular Component: multivesicular body; Golgi apparatus; cell surface; cytoplasmic vesicle membrane; integral to plasma membrane; axon; endoplasmic reticulum; late endosome; integral to membrane; plasma membrane; trans-Golgi network; endosome

Molecular Function: protein binding; enzyme binding; beta-amyloid binding; beta-aspartyl-peptidase activity; aspartic-type endopeptidase activity

Biological Process: beta-amyloid metabolic process; membrane protein ectodomain proteolysis; proteolysis