Western Blot (WB)
(Western blot analysis of B4GALT1 expression in HL60 (A), HEK293T (B), Jurkat (C) whole cell lysates.)
Rabbit B4GALT1 Polyclonal Antibody | anti-B4GALT1 antibody
Anti-B4GALT1 Antibody
Western Blot (WB)
(Western blot analysis of B4GALT1 expression in HL60 (A), HEK293T (B), Jurkat (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of B4GALT1 expression in HL60 (A), HEK293T (B), Jurkat (C) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of B4GALT1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of B4GALT1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. This gene is unique among the beta4GalT genes because it encodes an enzyme that participates both in glycoconjugate and lactose biosynthesis. For the first activity, the enzyme adds galactose to N-acetylglucosamine residues that are either monosaccharides or the nonreducing ends of glycoprotein carbohydrate chains. The second activity is restricted to lactating mammary tissues where the enzyme forms a heterodimer with alpha-lactalbumin to catalyze UDP-galactose + D-glucose <=> UDP + lactose. The two enzymatic forms result from alternate transcription initiation sites and post-translational processing. Two transcripts, which differ only at the 5' end, with approximate lengths of 4.1 kb and 3.9 kb encode the same protein. The longer transcript encodes the type II membrane-bound, trans-Golgi resident protein involved in glycoconjugate biosynthesis. The shorter transcript encodes a protein which is cleaved to form the soluble lactose synthase. [provided by RefSeq, Jul 2008]
Uniprot Description
B4GALT1: The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Defects in B4GALT1 are the cause of congenital disorder of glycosylation type 2D (CDG2D). CDGs are a family of severe inherited diseases caused by a defect in protein N- glycosylation. They are characterized by under-glycosylated serum proteins. These multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. Belongs to the glycosyltransferase 7 family. 2 isoforms of the human protein are produced by alternative initiation.
Protein type: EC 2.4.1.38; Cell adhesion; EC 2.4.1.90; Cell development/differentiation; EC 2.4.1.22; Glycan Metabolism - keratan sulfate biosynthesis; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; Carbohydrate Metabolism - galactose; Motility/polarity/chemotaxis; Membrane protein, integral; Glycan Metabolism - N-glycan biosynthesis; Cell surface; Transferase
Chromosomal Location of Human Ortholog: 9p13
Cellular Component: basolateral plasma membrane; brush border membrane; desmosome; external side of plasma membrane; extracellular space; filopodium; glycocalyx; Golgi apparatus; Golgi membrane; Golgi trans cisterna; integral to membrane; membrane; plasma membrane
Molecular Function: alpha-tubulin binding; beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity; beta-tubulin binding; cytoskeletal protein binding; galactosyltransferase activity; lactose synthase activity; manganese ion binding; N-acetyllactosamine synthase activity; protein homodimerization activity; protein kinase binding; UDP-galactosyltransferase activity
Biological Process: acute inflammatory response; angiogenesis involved in wound healing; binding of sperm to zona pellucida; branching morphogenesis of a tube; carbohydrate metabolic process; cell adhesion; cellular protein metabolic process; development of secondary sexual characteristics; epithelial cell development; extracellular matrix organization and biogenesis; galactose metabolic process; glycosaminoglycan metabolic process; keratan sulfate biosynthetic process; keratan sulfate metabolic process; lactose biosynthetic process; leukocyte migration; mammary gland development; multicellular organism reproduction; negative regulation of cell proliferation; Notch signaling pathway; oligosaccharide biosynthetic process; penetration of zona pellucida; positive regulation of apoptosis involved in mammary gland involution; positive regulation of epithelial cell proliferation involved in wound healing; post-translational protein modification; protein amino acid N-linked glycosylation; protein amino acid N-linked glycosylation via asparagine; regulation of acrosome reaction; regulation of cell motility; single fertilization
Disease: Congenital Disorder Of Glycosylation, Type Iid
Research Articles on B4GALT1
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Product Notes
The B4GALT1 b4galt1 (Catalog #AAA8242969) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-B4GALT1 Antibody reacts with Human, Mouse, Rat, Bovine, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's B4GALT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200). Researchers should empirically determine the suitability of the B4GALT1 b4galt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B4GALT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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