Human Beta-1,4-Galactosyltransferase 1 (b4GALT1) RTU ELISA Kit | b4GALT1 rtu elisa kit

Human Beta-1,4-Galactosyltransferase 1 (b4GALT1) ELISA Kit

Cat. Num. AAA457849

• Gene Names: B4GALT1; GT1; GTB; CDG2D; GGTB2; B4GAL-T1; beta4Gal-T1
• Reactivity: Human
• Synonyms: Beta-1; 4-Galactosyltransferase 1 (b4GALT1); Human Beta-1; 4-Galactosyltransferase 1 (b4GALT1) ELISA Kit; GTB; B4-GALT1; GGTB2; GT1; Beta4Gal-T1; Lactose Synthase A Protein; N-Acetyllactosamine Synthase; Nal synthase; beta-N-acetylglucosamine beta-1; 4-galactosyltransferase 1; b4GALT1 rtu elisa kit

• Reactivity: Human
• Sequence Length: 398

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 1.56-100ng/mL
• Sensitivity: 0.57ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for b4GALT1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to b4GALT1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to b4GALT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain b4GALT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of b4GALT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13929462
• NCBI GeneID: 2683
• NCBI Accession #: NP_001488.2
• NCBI GenBank Nucleotide #: NM_001497.3
• UniProt Accession #: P15291; Q12909; Q12910; Q12911; Q14456; Q14509; Q14523; B2R710; D3DRL2
• Molecular Weight: 42,538 Da
• NCBI Official Full Name: beta-1,4-galactosyltransferase 1
• NCBI Official Synonym Full Names: beta-1,4-galactosyltransferase 1
• NCBI Official Symbol: B4GALT1
• NCBI Official Synonym Symbols: GT1; GTB; CDG2D; GGTB2; B4GAL-T1; beta4Gal-T1
• NCBI Protein Information: beta-1,4-galactosyltransferase 1
• UniProt Protein Name: Beta-1,4-galactosyltransferase 1
• Protein Family: Beta-1,4-galactosyltransferase
• UniProt Gene Name: B4GALT1
• UniProt Synonym Gene Names: GGTB2; Beta-1,4-GalTase 1; Beta4Gal-T1; b4Gal-T1

NCBI Description

This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. This gene is unique among the beta4GalT genes because it encodes an enzyme that participates both in glycoconjugate and lactose biosynthesis. For the first activity, the enzyme adds galactose to N-acetylglucosamine residues that are either monosaccharides or the nonreducing ends of glycoprotein carbohydrate chains. The second activity is restricted to lactating mammary tissues where the enzyme forms a heterodimer with alpha-lactalbumin to catalyze UDP-galactose + D-glucose <=> UDP + lactose. The two enzymatic forms result from alternate transcription initiation sites and post-translational processing. Two transcripts, which differ only at the 5' end, with approximate lengths of 4.1 kb and 3.9 kb encode the same protein. The longer transcript encodes the type II membrane-bound, trans-Golgi resident protein involved in glycoconjugate biosynthesis. The shorter transcript encodes a protein which is cleaved to form the soluble lactose synthase. [provided by RefSeq, Jul 2008]

Uniprot Description

The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.