Human keratin 20 ELISA Kit | KRT20 elisa kit

Human cytokeratin 20, CK20 ELISA Kit

Cat. Num. AAA700164

• Gene Names: KRT20; K20; CD20; CK20; CK-20; KRT21
• Reactivity: Human
• Synonyms: keratin 20; Human cytokeratin 20; CK20 ELISA Kit; CD20; CK20; K20; KRT21; MGC35423; cytokeratin 20; keratin; type I cytoskeletal 20; KRT20 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human CK20. No significant cross-reactivity or interference between human CK20 and analogues was observed.
• Sequence Length: 424

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 0.625 ng/ml -40 ng/ml.
• Sensitivity: The minimum detectable dose of human CK20 is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KRT20 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CK20 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CK20 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CK20 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CK20 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 27894337
• NCBI GeneID: 54474
• NCBI Accession #: NP_061883.1
• NCBI GenBank Nucleotide #: NM_019010.2
• UniProt Accession #: P35900; B2R6W7
• Molecular Weight: 48,487 Da
• NCBI Official Full Name: keratin, type I cytoskeletal 20
• NCBI Official Synonym Full Names: keratin 20
• NCBI Official Symbol: KRT20
• NCBI Official Synonym Symbols: K20; CD20; CK20; CK-20; KRT21
• NCBI Protein Information: keratin, type I cytoskeletal 20; keratin-20; protein IT; cytokeratin 20; cytokeratin-20
• UniProt Protein Name: Keratin, type I cytoskeletal 20
• Protein Family: Keratin
• UniProt Gene Name: KRT20
• UniProt Synonym Gene Names: CK-20; K20
• UniProt Entry Name: K1C20_HUMAN

NCBI Description

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine 2 PublicationsManual assertion based on experiment in:Ref.4