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Western Blot (WB) (Western blot analysis of extracts from HeLa cells, K562 cells, HepG2 cells and HuvEC cells, using B4GALNT1 antibody.)

Rabbit anti-Human B4GALNT1 Polyclonal Antibody | anti-B4GALNT1 antibody

B4GALNT1 Antibody

Gene Names
B4GALNT1; GALGT; SPG26; GALNACT; GalNAc-T
Reactivity
Human
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
B4GALNT1; Polyclonal Antibody; B4GALNT1 Antibody; Beta-1; 4 N-acetylgalactosaminyltransferase 1; (N-acetylneuraminyl)-galactosylglucosylceramide; GM2/GD2 synthase; GalNAc-T; anti-B4GALNT1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total B4GALNT1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
478
Applicable Applications for anti-B4GALNT1 antibody
Western Blot (WB)
Application Notes
Western blotting: 1:500~1:3000
Immunogen Type
Peptide
Immunogen Description
Synthesized peptide derived from internal of human B4GALNT1.
Target Name
B4GALNT1
Preparation and Storage
Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells, K562 cells, HepG2 cells and HuvEC cells, using B4GALNT1 antibody.)

Western Blot (WB) (Western blot analysis of extracts from HeLa cells, K562 cells, HepG2 cells and HuvEC cells, using B4GALNT1 antibody.)
Related Product Information for anti-B4GALNT1 antibody
Involved in the biosynthesis of gangliosides GM2, GD2 and GA2.Nagata Y., J. Biol. Chem. 267:12082-12089(1992).Nagata Y., J. Biol. Chem. 269:7045-7045(1994).Li J., J. Biol. Chem. 275:41476-41486(2000).
Product Categories/Family for anti-B4GALNT1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
35,471 Da
NCBI Official Full Name
beta-1,4 N-acetylgalactosaminyltransferase 1 isoform 2
NCBI Official Synonym Full Names
beta-1,4-N-acetyl-galactosaminyl transferase 1
NCBI Official Symbol
B4GALNT1
NCBI Official Synonym Symbols
GALGT; SPG26; GALNACT; GalNAc-T
NCBI Protein Information
beta-1,4 N-acetylgalactosaminyltransferase 1
UniProt Protein Name
Beta-1,4 N-acetylgalactosaminyltransferase 1
UniProt Gene Name
B4GALNT1
UniProt Synonym Gene Names
GALGT; SIAT2
UniProt Entry Name
B4GN1_HUMAN

NCBI Description

GM2 and GD2 gangliosides are sialic acid-containing glycosphingolipids. GalNAc-T is the enzyme involved in the biosynthesis of G(M2) and G(D2) glycosphingolipids. GalNAc-T catalyzes the transfer of GalNAc into G(M3) and G(D3) by a beta-1,4 linkage, resulting in the synthesis of G(M2) and G(D2), respectively. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2013]

Uniprot Description

B4GALNT1: Involved in the biosynthesis of gangliosides GM2, GD2 and GA2. Belongs to the glycosyltransferase 2 family.

Protein type: Transferase; EC 2.4.1.92; Membrane protein, integral; Glycan Metabolism - glycosphingolipid biosynthesis - ganglio series

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: membrane; plasma membrane; integral to Golgi membrane

Molecular Function: (N-acetylneuraminyl)-galactosylglucosylceramide N-acetylgalactosaminyltransferase activity

Biological Process: sequestering of lipid; carbohydrate metabolic process; lipid glycosylation; protein amino acid glycosylation; glycosphingolipid metabolic process; spermatogenesis; ganglioside biosynthetic process

Research Articles on B4GALNT1

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Product Notes

The B4GALNT1 b4galnt1 (Catalog #AAA9402449) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The B4GALNT1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's B4GALNT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blotting: 1:500~1:3000. Researchers should empirically determine the suitability of the B4GALNT1 b4galnt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B4GALNT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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