Rabbit anti-Human, Mouse B3GNT7 Polyclonal Antibody | anti-B3GNT7 antibody

B3GNT7, ID (B3GNT7, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 7) (FITC)

Cat. Num. AAA6277176

• Gene Names: B3GNT7; beta3GnT7
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: B3GNT7; Polyclonal Antibody; ID (B3GNT7; UDP-GlcNAc:betaGal beta-1; 3-N-acetylglucosaminyltransferase 7) (FITC); 3-N-acetylglucosaminyltransferase 7; anti-B3GNT7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-B3GNT7 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: B3GNT7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 262~291 amino acids from the Central region of human B3GNT7.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of B3GNT7 Antibody (Center) in mouse heart tissue lysates (35ug/lane). B3GNT7 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(B3GNT7 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the B3GNT7 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-B3GNT7 antibody

B3GNT7 is a glycosyltransferase which may play a role in elongation of the keratan sulfate carbohydrate backbone.

Product Categories/Family for anti-B3GNT7 antibody

Antibodies; Enzymes; Glycosyltransferase

References

Kitayama, K., et al. J. Biol. Chem. 282(41):30085-30096(2007). Seko, A., et al. FEBS Lett. 556 (1-3), 216-220 (2004). Kataoka, K., et al. Biochem. Biophys. Res. Commun. 294(4):843-848(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 21687139
• NCBI GeneID: 93010
• NCBI Accession #: NP_660279.1
• NCBI GenBank Nucleotide #: NM_145236.2
• UniProt Accession #: Q8NFL0; B3KWY4; B7WNP0
• Molecular Weight: 45,987 Da
• NCBI Official Full Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 7
• NCBI Official Synonym Full Names: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 7
• NCBI Official Symbol: B3GNT7
• NCBI Official Synonym Symbols: beta3GnT7
• NCBI Protein Information: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 7; BGnT-7; beta3Gn-T7; beta-1,3-Gn-T7; beta 1,3-N-acetylglucosaminyltransferase 7
• UniProt Protein Name: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 7
• Protein Family: UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase
• UniProt Gene Name: B3GNT7
• UniProt Synonym Gene Names: BGnT-7; Beta-1,3-Gn-T7; Beta-1,3-N-acetylglucosaminyltransferase 7; Beta3Gn-T7
• UniProt Entry Name: B3GN7_HUMAN

Uniprot Description

B3GNT7: May be involved in keratane sulfate biosynthesis. Transfers N-acetylgalactosamine on to keratan sulfate-related glycans. May play a role in preventing cells from migrating out of the original tissues and invading surrounding tissues. Belongs to the glycosyltransferase 31 family.

Protein type: EC 2.4.1.-; Membrane protein, integral; Glycan Metabolism - keratan sulfate biosynthesis; Transferase

Chromosomal Location of Human Ortholog: 2q37.1|2q37.1

Cellular Component: Golgi membrane; integral to membrane

Molecular Function: galactosyltransferase activity

Biological Process: keratan sulfate metabolic process; protein amino acid O-linked glycosylation; cellular protein metabolic process; glycosaminoglycan metabolic process; O-glycan processing; carbohydrate metabolic process; keratan sulfate biosynthetic process; pathogenesis; post-translational protein modification