Typical Testing Data/Standard Curve (for reference only)
Human Deubiquitinating enzyme ELISA Kit | DUB elisa kit
Human Deubiquitinating enzyme ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: DUB ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-DUB antibody and an DUB-HRP conjugate. The assay sample and buffer are incubated together with DUB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DUB concentration since DUB from samples and DUB-HRP conjugate compete for the anti-DUB antibody binding site. Since the number of sites is limited, as more sites are occupied by DUB from the sample, fewer sites are left to bind DUB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DUB concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the ubiquitin specific protease (USP) family of deubiquitinating enzymes. USP enzymes play critical roles in ubiquitin-dependent processes through polyubiquitin chain disassembly and hydrolysis of ubiquitin-substrate bonds. The encoded protein associates with the COP9 signalosome, and also plays a role in transforming growth factor beta signalling through deubiquitination of receptor-activated SMAD transcription factors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 2. [provided by RefSeq, Nov 2011]
Uniprot Description
USP15: Protease that degrades 'Lys-48'-linked polyubiquitin chains. Protects target proteins against proteasomal degradation. Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway. Belongs to the peptidase C19 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.19.12; Ubiquitin-specific protease; Protease; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 12q14
Cellular Component: cytoplasm; nucleus
Molecular Function: identical protein binding; protein binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; SMAD binding; transforming growth factor beta receptor binding
Biological Process: BMP signaling pathway; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; transforming growth factor beta receptor signaling pathway; negative regulation of transforming growth factor beta receptor signaling pathway
Research Articles on DUB
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Product Notes
The Human DUB usp15 (Catalog #AAA727833) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA727833 ELISA Kit recognizes Human DUB. It is sometimes possible for the material contained within the vial of "Deubiquitinating enzyme, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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