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Western Blot (WB) (Western blot analysis of B3GNT6 Antibody (Center) in mouse brain tissue lysates (35ug/lane). B3GNT6 (arrow) was detected using the purified Pab.)

Rabbit anti-Human, mouse B3GNT6 Polyclonal Antibody | anti-B3GNT6 antibody

B3GNT6 Antibody (Center)

Gene Names
B3GNT6; BGnT-6; B3Gn-T6; beta3Gn-T6; beta-1,3-Gn-T6
Reactivity
Human, mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
B3GNT6; Polyclonal Antibody; B3GNT6 Antibody (Center); UDP-GlcNAc:betaGal beta-1; 3-N-acetylglucosaminyltransferase 6; BGnT-6; Beta-1; 3-Gn-T6; Beta3Gn-T6; 241-; Core 3 synthase; anti-B3GNT6 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This B3GNT6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-173 amino acids from the Central region of human B3GNT6.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
146-173
Sequence Length
384
Applicable Applications for anti-B3GNT6 antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of B3GNT6 Antibody (Center) in mouse brain tissue lysates (35ug/lane). B3GNT6 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of B3GNT6 Antibody (Center) in mouse brain tissue lysates (35ug/lane). B3GNT6 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(B3GNT6 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the B3GNT6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (B3GNT6 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the B3GNT6 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Product Categories/Family for anti-B3GNT6 antibody
References
Lee, S.H., et al. J. Biol. Chem. 284(25):17157-17169(2009)
Iwai, T., et al. Proc. Natl. Acad. Sci. U.S.A. 102(12):4572-4577(2005)
Iwai, T., et al. J. Biol. Chem. 277(15):12802-12809(2002)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42748
NCBI Official Full Name
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6
NCBI Official Synonym Full Names
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6
NCBI Official Symbol
B3GNT6
NCBI Official Synonym Symbols
BGnT-6; B3Gn-T6; beta3Gn-T6; beta-1,3-Gn-T6
NCBI Protein Information
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6
UniProt Protein Name
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6
UniProt Gene Name
B3GNT6
UniProt Synonym Gene Names
BGnT-6; Beta-1,3-Gn-T6; Beta-1,3-N-acetylglucosaminyltransferase 6; Beta3Gn-T6
UniProt Entry Name
B3GN6_HUMAN

Uniprot Description

B3GNT6: Beta-1,3-N-acetylglucosaminyltransferase that synthesizes the core 3 structure of the O-glycan, an important precursor in the biosynthesis of mucin-type glycoproteins. Plays an important role in the synthesis of mucin-type O-glycans in digestive organs. Belongs to the glycosyltransferase 31 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.4.1.-; Membrane protein, integral; Glycan Metabolism - O-glycan biosynthesis; Transferase

Chromosomal Location of Human Ortholog: 11q13.4

Cellular Component: Golgi membrane; membrane; integral to membrane

Molecular Function: beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase activity; galactosyltransferase activity

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; glycoprotein biosynthetic process; O-glycan processing, core 3; post-translational protein modification

Research Articles on B3GNT6

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Product Notes

The B3GNT6 b3gnt6 (Catalog #AAA9210849) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 146-173. The B3GNT6 Antibody (Center) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's B3GNT6 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the B3GNT6 b3gnt6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B3GNT6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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