Western Blot (WB)
(B3GNT5 Antibody (Center) western blot analysis in 293,MDA-MB435,HL-60 cell line lysates (35ug/lane).This demonstrates the B3GNT5 antibody detected the B3GNT5 protein (arrow).)
Rabbit anti-Human, Mouse B3GNT5 Polyclonal Antibody | anti-B3GNT5 antibody
B3GNT5, ID (B3GNT5, Lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase, Lactotriaosylceramide synthase, UDP-GlcNAc:beta-Gal beta-1,3-N-acetylglucosaminyltransferase 5) (Biotin)
Gene Names
B3GNT5; B3GN-T5; beta3Gn-T5
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
B3GNT5; Polyclonal Antibody; ID (B3GNT5; Lactosylceramide 1; 3-N-acetyl-beta-D-glucosaminyltransferase; Lactotriaosylceramide synthase; UDP-GlcNAc:beta-Gal beta-1; 3-N-acetylglucosaminyltransferase 5) (Biotin); 3-N-acetylglucosaminyltransferase 5; anti-B3GNT5 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-B3GNT5 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
B3GNT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124~154 amino acids from the Central region of human B3GNT5.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(B3GNT5 Antibody (Center) western blot analysis in 293,MDA-MB435,HL-60 cell line lysates (35ug/lane).This demonstrates the B3GNT5 antibody detected the B3GNT5 protein (arrow).)
Western Blot (WB)
(B3GNT5 Antibody (Center) western blot analysis in 293,MDA-MB435,HL-60 cell line lysates (35ug/lane).This demonstrates the B3GNT5 antibody detected the B3GNT5 protein (arrow).)
Western Blot (WB)
(Western blot analysis of B3GNT5 Antibody (Center) in mouse liver tissue lysates (35ug/lane). B3GNT5 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of B3GNT5 Antibody (Center) in mouse liver tissue lysates (35ug/lane). B3GNT5 (arrow) was detected using the purified Pab.)
Related Product Information for anti-B3GNT5 antibody
B3GNT5 is a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II membrane protein. It exhibits strong activity to transfer GlcNAc to glycolipid substrates and is identified as the most likely candidate for lactotriaosylceramide synthase. This enzyme is essential for the expression of Lewis X epitopes on glycolipids.
Product Categories/Family for anti-B3GNT5 antibody
References
Marcos, N.T., et al. J. Clin. Invest. 118(6):2325-2336(2008). Togayachi, A., et al. J. Biol. Chem. 276(25):22032-22040(2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,053 Da
NCBI Official Full Name
lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase
NCBI Official Synonym Full Names
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5
NCBI Official Symbol
B3GNT5
NCBI Official Synonym Symbols
B3GN-T5; beta3Gn-T5
NCBI Protein Information
lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase
UniProt Protein Name
Lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase
Protein Family
UniProt Gene Name
B3GNT5
UniProt Synonym Gene Names
Lc(3)Cer synthase; Lc3 synthase; BGnT-5; Beta-1,3-Gn-T5; Beta-1,3-N-acetylglucosaminyltransferase 5; Beta3Gn-T5
NCBI Description
This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II membrane protein. It exhibits strong activity to transfer GlcNAc to glycolipid substrates and is identified as the most likely candidate for lactotriaosylceramide synthase. This enzyme is essential for the expression of Lewis X epitopes on glycolipids. [provided by RefSeq, Jul 2008]
Uniprot Description
Beta-1,3-N-acetylglucosaminyltransferase that plays a key role in the synthesis of lacto- or neolacto-series carbohydrate chains on glycolipids, notably by participating in biosynthesis of HNK-1 and Lewis X carbohydrate structures. Has strong activity toward lactosylceramide (LacCer) and neolactotetraosylceramide (nLc4Cer; paragloboside), resulting in the synthesis of Lc3Cer and neolactopentaosylceramide (nLc5Cer), respectively. Probably plays a central role in regulating neolacto-series glycolipid synthesis during embryonic development.
Research Articles on B3GNT5
Similar Products
Product Notes
The B3GNT5 b3gnt5 (Catalog #AAA6277153) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The B3GNT5, ID (B3GNT5, Lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase, Lactotriaosylceramide synthase, UDP-GlcNAc:beta-Gal beta-1,3-N-acetylglucosaminyltransferase 5) (Biotin) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's B3GNT5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the B3GNT5 b3gnt5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B3GNT5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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