Human Kelch Like ECH Associated Protein 1 (KEAP1) ELISA Kit | KEAP1 elisa kit

Human Kelch Like ECH Associated Protein 1 (KEAP1) ELISA Kit

Cat. Num. AAA2706970

• Gene Names: KEAP1; INrf2; KLHL19
• Reactivity: Human
• Synonyms: Kelch Like ECH Associated Protein 1 (KEAP1); Human Kelch Like ECH Associated Protein 1 (KEAP1) ELISA Kit; INrf2; KLHL19; Cytosolic inhibitor of Nrf2; Kelch-like protein 19; KEAP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Kelch Like ECH Associated Protein 1 (KEAP1).; No significant cross-reactivity or interference between Kelch Like ECH Associated Protein 1 (KEAP1) and analogues was observed.
• Sequence Length: 624

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.056ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kelch Like ECH Associated Protein 1 (KEAP1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kelch Like ECH Associated Protein 1 (KEAP1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KEAP1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kelch Like ECH Associated Protein 1 (KEAP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kelch Like ECH Associated Protein 1 (KEAP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kelch Like ECH Associated Protein 1 (KEAP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kelch Like ECH Associated Protein 1 (KEAP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 22027642
• NCBI GeneID: 9817
• NCBI Accession #: NP_036421.2
• NCBI GenBank Nucleotide #: NM_012289.3
• UniProt Accession #: Q14145; Q6LEP0; Q8WTX1; Q9BPY9; B3KPD5
• Molecular Weight: 69,666 Da
• NCBI Official Full Name: kelch-like ECH-associated protein 1
• NCBI Official Synonym Full Names: kelch like ECH associated protein 1
• NCBI Official Symbol: KEAP1
• NCBI Official Synonym Symbols: INrf2; KLHL19
• NCBI Protein Information: kelch-like ECH-associated protein 1
• UniProt Protein Name: Kelch-like ECH-associated protein 1
• Protein Family: Kelch-like ECH-associated protein
• UniProt Gene Name: KEAP1
• UniProt Synonym Gene Names: INRF2; KIAA0132; KLHL19; INrf2

NCBI Description

This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

KEAP1: a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Has tumor suppressor activity. Retains NRF2, BPTF and PGAM5 in the cytosol. Targets NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and of antioxidant response element-mediated detoxifying enzyme gene expression. Somatic mutations of KEAP1 or NRF2 commonly occur in solid cancers, irrespective of histological type, resulting in the constitutive transcription of cytoprotective genes. Genetic disruption of the KEAP1/CUL3 E3 ubiquitin ligase complex leads to the activation of the NF-kappaB pathway in lung cancer. Alternative activation of the cytoprotective NRF2 pathway exist in the absence of mutations in KEAP1 and HRF2: WTX, PALB2, SQSTM1, and DPP3 can competitively bind KEAP1. These proteins and others that bind KEAP1 contain an ¿ETGE¿ amino acid motif, which matches the KEAP1 interaction motif found in NRF2. Binding of these ¿ETGE¿-containing proteins can displace NRF2, freeing it to translocate to the nucleus and implement its cytoprotective transcriptional pathway. The DPP3 gene is frequently overexpressed in squamous cell lung carcinomas with high wt NRF2 activity. Its expression level significantly correlates with the response rate and progression-free survival of platinum-based first-line chemotherapy. May be a useful biomarker for predicting the chemosensitivity of patients with advanced-stage NSCLC. Broadly expressed, with highest levels in skeletal muscle. Ubiquitination and subsequent degradation of PGAM5 is inhibited by oxidative stress and sulforaphane.

Protein type: Endoplasmic reticulum; Motility/polarity/chemotaxis; Transcription regulation; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: actin filament; cytoplasm; cytosol; endoplasmic reticulum; microtubule organizing center; midbody; nucleoplasm; protein complex

Molecular Function: protein binding; protein homodimerization activity; transcription factor binding; ubiquitin-protein transferase activity

Biological Process: cytoplasmic sequestering of transcription factor; in utero embryonic development; negative regulation of transcription factor activity; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; post-translational protein modification; proteasomal ubiquitin-independent protein catabolic process; protein deubiquitination; protein ubiquitination; regulation of epidermal cell differentiation; transcription, DNA-dependent