Rabbit anti-Human, rat AXL Polyclonal Antibody | anti-AXL antibody

AXL Antibody (C-term)

Cat. Num. AAA9203725

• Gene Names: AXL; ARK; UFO; JTK11; Tyro7
• Reactivity: Human, rat
• Applications: Western Blot, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: AXL; Polyclonal Antibody; AXL Antibody (C-term); Tyrosine-protein kinase receptor UFO; AXL oncogene; UFO; anti-AXL antibody

• Host: Rabbit
• Reactivity: Human, rat
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This AXL antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 838-872 amino acids from the C-terminal region of human AXL.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Length: 885

• Applicable Applications for anti-AXL antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:2000
• Crown Antibody: Yes
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-AXL Antibody (C-term) at 1:2000 dilutionLane 1: NCI-H1299 whole cell lysatesLane 2: Hela whole cell lysatesLane 3: L6 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 98 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-AXL antibody

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of PI3- kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TENC1. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response. In case of filovirus infection, seems to function as a cell entry factor.

Product Categories/Family for anti-AXL antibody

Crown Antibodies

References

Partanen J.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:8913-8917(1990).
O'Bryan J.P.,et al.Mol. Cell. Biol. 11:5016-5031(1991).
Janssen J.W.G.,et al.Oncogene 6:2113-2120(1991).
Grimwood J.,et al.Nature 428:529-535(2004).
Lee S.-T.,et al.Oncogene 8:3403-3410(1993).

NCBI and Uniprot Product Information

• NCBI GI #: 21536468
• NCBI GeneID: 558
• NCBI Accession #: NP_001690.2
• NCBI GenBank Nucleotide #: NM_001699.5
• UniProt Accession #: P30530; Q8N5L2; Q9UD27
• Molecular Weight: 98336
• NCBI Official Full Name: tyrosine-protein kinase receptor UFO isoform 2
• NCBI Official Synonym Full Names: AXL receptor tyrosine kinase
• NCBI Official Symbol: AXL
• NCBI Official Synonym Symbols: ARK; UFO; JTK11; Tyro7
• NCBI Protein Information: tyrosine-protein kinase receptor UFO
• UniProt Protein Name: Tyrosine-protein kinase receptor UFO
• Protein Family: Tyrosine-protein kinase receptor
• UniProt Gene Name: AXL
• UniProt Synonym Gene Names: UFO
• UniProt Entry Name: UFO_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]

Uniprot Description

AXL: a receptor tyrosine kinase that may function as a signal transducer between specific cell types of mesodermal origin. Interacts with SKP1. Overexpression in tissue culture causes oncogenic transformation. Overexpressed in several cancers including thyroid, ovarian, gastric, ER+ breast cancer and acute myeloid leukemia, where it is associated with poor prognosis. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, TK; EC 2.7.10.1; Oncoprotein; Membrane protein, integral; Protein kinase, tyrosine (receptor); TK group; Axl family

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: extracellular space; cell surface; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; protein heterodimerization activity; phosphatidylserine binding; protein-tyrosine kinase activity; phosphoinositide 3-kinase binding; transmembrane receptor protein tyrosine kinase activity; ATP binding; myosin heavy chain binding

Biological Process: peptidyl-tyrosine phosphorylation; cell maturation; neuron migration; signal transduction; positive regulation of natural killer cell differentiation; erythrocyte homeostasis; ovulation cycle; natural killer cell differentiation; negative regulation of interferon-gamma production; protein kinase B signaling cascade; negative regulation of neuron apoptosis; inflammatory response; secretion by cell; forebrain cell migration; negative regulation of lymphocyte activation; platelet activation; organ regeneration; vagina development; negative regulation of tumor necrosis factor production; phagocytosis; positive regulation of protein kinase B signaling cascade; cellular response to extracellular stimulus; innate immune response; spermatogenesis; blood vessel remodeling; vascular endothelial growth factor receptor signaling pathway; positive regulation of cytokine and chemokine mediated signaling pathway; apoptotic cell clearance