Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Axis Inhibition Protein (AXIN) Polyclonal Antibody | anti-AXIN antibody
Polyclonal Antibody to Axis Inhibition Protein (AXIN)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS-KQQAHES SRPLSIERPG AVHPWVSAQL RNSVQPSHLF IQDPTMPPNP APNPLTQLEE ARRRLEEEEK RANKLPSKQR YVQAVMQRGR TCVRPACAPV LSVVPAVSDL ELSETETKSQ RKAGGGSAPP CDSIVVAYYF CGEPIPYRTL VRGRAVTLGQ FKELLTKKGS YRYYFKKVSD EFDCGVVFEE VREDEAVLPV FEEKIIGKVE KVD
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
NCBI and Uniprot Product Information
Uniprot Description
axin 1: is a negative regulator of the Wnt pathway, which is critical in stem cell signaling, morphogenesis, the mesenchymal-epithelial transition, and many cancers. Axin-1 functions as a tumor suppressor. Probably facilitates the phosphorylation of beta-catenin and APC by GSK3B, leading to their ubiquitination and subsequent proteolysis. Wild-type axin 1 can induce apoptosis in hepatocellular and colorectal cancer cells. Is downregulated during progression of esophageal squamous cell carcinoma. Mutation of the axin-1 gene is associated with hepatocellular carcinoma, anaplastic thyroid cancer, medulloblastoma and colorectal cancer. May have a role in oncogenesis in Hodgkin lymphoma. Axin1/2 mediate cross-talk between TGF-beta and Wnt signaling pathways.
Protein type: Adaptor/scaffold; Tumor suppressor
Chromosomal Location of Human Ortholog: 17 A3.3|17 13.07 cM
Cellular Component: beta-catenin destruction complex; cell cortex; cell periphery; cytoplasm; cytoplasmic microtubule; cytoplasmic vesicle; Golgi apparatus; lateral plasma membrane; membrane; nucleus; perinuclear region of cytoplasm; plasma membrane; postsynaptic density; protein complex; Wnt signalosome
Molecular Function: armadillo repeat domain binding; beta-catenin binding; enzyme binding; GTPase activator activity; I-SMAD binding; identical protein binding; p53 binding; protein binding; protein C-terminus binding; protein complex scaffold activity; protein domain specific binding; protein homodimerization activity; protein kinase binding; protein self-association; R-SMAD binding; receptor binding; receptor signaling complex scaffold activity; signal transducer activity; SMAD binding; ubiquitin protein ligase binding
Biological Process: activation of JNK activity; activation of protein kinase activity; adult walking behavior; apoptosis; axial mesoderm development; axial mesoderm formation; cellular protein complex assembly; cytoplasmic microtubule organization; determination of left/right symmetry; dorsal/ventral axis specification; dorsal/ventral pattern formation; embryonic eye morphogenesis; erythrocyte homeostasis; forebrain anterior/posterior pattern specification; genetic imprinting; hemoglobin metabolic process; in utero embryonic development; multicellular organism development; muscle cell development; negative regulation of canonical Wnt signaling pathway; negative regulation of fat cell differentiation; negative regulation of gene expression; negative regulation of protein metabolic process; negative regulation of Wnt signaling pathway; neurological system process; nucleocytoplasmic transport; olfactory placode formation; optic placode formation; positive regulation of JNK cascade; positive regulation of JUN kinase activity; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-threonine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein catabolic process; positive regulation of protein kinase activity; positive regulation of protein phosphorylation; positive regulation of protein ubiquitination; positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process; positive regulation of transcription, DNA-templated; positive regulation of transforming growth factor beta receptor signaling pathway; positive regulation of ubiquitin-protein transferase activity; protein catabolic process; protein homooligomerization; protein polyubiquitination; regulation of protein amino acid phosphorylation; sensory perception of sound; signal transduction; Wnt receptor signaling pathway involved in forebrain neuron fate commitment; Wnt receptor signaling pathway through beta-catenin; Wnt signaling pathway
Research Articles on AXIN
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Product Notes
The AXIN axin1 (Catalog #AAA2006981) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Axis Inhibition Protein (AXIN) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Axis Inhibition Protein (AXIN) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the AXIN axin1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS-KQQAH ES SRPLSIERPG AVHPWVSAQL RNSVQPSHLF IQDPTMPPNP APNPLTQLEE ARRRLEEEEK RANKLPSKQR YVQAVMQRGR TCVRPACAPV LSVVPAVSDL ELSETETKSQ RKAGGGSAPP CDSIVVAYYF CGEPIPYRTL VRGRAVTLGQ FKELLTKKGS YRYYFKKVSD EFDCGVVFEE VREDEAVLPV FEEKIIGKVE KVD. It is sometimes possible for the material contained within the vial of "Axis Inhibition Protein (AXIN), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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