Rabbit AXIN2 / Axin 2 Polyclonal Antibody | anti-AXIN2 antibody

Rabbit Polyclonal to Human AXIN2 / Axin 2

Cat. Num. AAA243754

• Gene Names: AXIN2; AXIL; ODCRCS
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence, ELISA
• Purity: Peptide Column Affinity Chromatography
• Synonyms: AXIN2 / Axin 2; Polyclonal Antibody; Rabbit Polyclonal to Human AXIN2 / Axin 2; Anti-AXIN2 / Axin 2 Antibody (C-Terminus) IHC-plus; AXIN2; AXIL; Axin-2; Axis inhibition protein 2; Axin 2; Axin-like protein; Conductin; Human AXIN2; anti-AXIN2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: AXIN2 antibody is predicted to not cross-react with AXIN1. At least four isoforms of AXIN2 are known to exist; this antibody will recognize all four.
• Purity/Purification: Peptide Column Affinity Chromatography
• Form/Format: PBS, 0.02% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 843

• Applicable Applications for anti-AXIN2 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: IHC-P: (5 µg/ml); WB: (1 - 2 µg/ml); ; AXIN2 antibody can be used for detection of AXIN2 by Western blot at 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested. Western Blot: 85, 93 kDa
• Target: AXIN/ Axin 2
• Immunogen: AXIN2/Axin 2 antibody was raised againist a 20 AA synthetic peptide near the C-Terminus of human AXIN2. The immunogen is located within aa 780-830 of AXIN2.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-AXIN2 / AXIL antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-AXIN2 / AXIL antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of AXIN2 in Rat Lung cells with AXIN2 antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot analysis of AXIN2 in mouse lung lysate with AXIN2 antibody at 1 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 195927059
• NCBI GeneID: 8313
• NCBI Accession #: NP_004646.3
• NCBI GenBank Nucleotide #: NM_004655.3
• UniProt Accession #: Q9Y2T1; Q3MJ88; Q9H3M6; Q9UH84
• NCBI Official Full Name: axin-2
• NCBI Official Synonym Full Names: axin 2
• NCBI Official Symbol: AXIN2
• NCBI Official Synonym Symbols: AXIL; ODCRCS
• NCBI Protein Information: axin-2; conductin; axin-like protein; axis inhibition protein 2
• UniProt Protein Name: Axin-2
• Protein Family: Axin
• UniProt Gene Name: AXIN2
• UniProt Synonym Gene Names: Axil
• UniProt Entry Name: AXIN2_HUMAN

NCBI Description

The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair. [provided by RefSeq, Jul 2008]

Uniprot Description

axin 2: is a negative regulator of the Wnt pathway, which is critical in stem cell signaling, morphogenesis, the mesenchymal-epithelial transition, and many cancers. Axin functions as a tumor suppressor. Probably facilitates the phosphorylation of beta-catenin and APC by GSK3B, leading to their ubiquitination and subsequent proteolysis. Is downregulated during progression of esophageal squamous cell carcinoma. Axin1/2 mediate cross-talk between TGF-beta and Wnt signaling pathways.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q24.1

Cellular Component: microtubule cytoskeleton; nucleoplasm; Golgi apparatus; centrosome; cytoplasmic microtubule; postsynaptic density; cytoplasmic membrane-bound vesicle; cytoplasm; plasma membrane; cell cortex; nucleus; beta-catenin destruction complex; cytosol

Molecular Function: protein binding; enzyme binding; ubiquitin protein ligase binding; beta-catenin binding; protein kinase binding; GTPase activator activity

Biological Process: odontogenesis; negative regulation of cell proliferation; cell proliferation; intramembranous ossification; regulation of mismatch repair; mRNA stabilization; negative regulation of osteoblast differentiation; dorsal/ventral axis specification; positive regulation of protein amino acid phosphorylation; maintenance of DNA repeat elements; bone mineralization; positive regulation of GTPase activity

Disease: Oligodontia-colorectal Cancer Syndrome