Rabbit anti-Human AXIN1 Polyclonal Antibody | anti-AXIN1 antibody

AXIN1, ID (AXIN1, AXIN, Axin-1, Axis inhibition protein 1) (APC)

Cat. Num. AAA6277009

• Gene Names: AXIN1; AXIN; PPP1R49
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: AXIN1; Polyclonal Antibody; ID (AXIN1; AXIN; Axin-1; Axis inhibition protein 1) (APC); Axis inhibition protein 1; anti-AXIN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AXIN1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-AXIN1 antibody: Western Blot (WB), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 330-359 in the Central region of human AXIN1, UniProt Accession #O15169. Species sequence homology: mouse, rat
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis in MDA-MB453 cell line lysates (35ug/lane) usingMBS6000898. This demonstrates MBS6000898 detected the AXIN1 protein (arrow).)

Related Product Information for anti-AXIN1 antibody

This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Two transcript variants encoding distinct isoforms have been identified for this gene.

Product Categories/Family for anti-AXIN1 antibody

Antibodies; Wnt Proteins

References

1. Sue Ng, S., et al. Biol. Chem. 391 (2-3), 171-180 (2010). 2. Yang, L.H., et al. Mol. Cancer 9, 25 (2010). 3. Wooten, E.C., et al. PLoS ONE 5 (1), E8830 (2010). 4. Kameoka, M., et al. AIDS Res. Hum. Retroviruses 25(10):1005-1011(2009). 5. Li, Q., et al. Nat. Cell Biol. 11(9):1128-1134(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 27501450
• NCBI GeneID: 8312
• NCBI Accession #: NP_003493.1
• NCBI GenBank Nucleotide #: NM_003502.3
• UniProt Accession #: O15169; Q4TT26; Q4TT27; Q86YA7; Q8WVW6; Q96S28
• Molecular Weight: 862
• NCBI Official Full Name: axin-1 isoform a
• NCBI Official Synonym Full Names: axin 1
• NCBI Official Symbol: AXIN1
• NCBI Official Synonym Symbols: AXIN; PPP1R49
• NCBI Protein Information: axin-1; axis inhibitor 1; fused, mouse, homolog of; axis inhibition protein 1; protein phosphatase 1, regulatory subunit 49
• UniProt Protein Name: Axin-1
• Protein Family: Axin
• UniProt Gene Name: AXIN1
• UniProt Synonym Gene Names: AXIN; hAxin
• UniProt Entry Name: AXIN1_HUMAN

Uniprot Description

axin 1: is a negative regulator of the Wnt pathway, which is critical in stem cell signaling, morphogenesis, the mesenchymal-epithelial transition, and many cancers. Axin-1 functions as a tumor suppressor. Probably facilitates the phosphorylation of beta-catenin and APC by GSK3B, leading to their ubiquitination and subsequent proteolysis. Wild-type axin 1 can induce apoptosis in hepatocellular and colorectal cancer cells. Is downregulated during progression of esophageal squamous cell carcinoma. Mutation of the axin-1 gene is associated with hepatocellular carcinoma, anaplastic thyroid cancer, medulloblastoma and colorectal cancer. May have a role in oncogenesis in Hodgkin lymphoma. Axin1/2 mediate cross-talk between TGF-beta and Wnt signaling pathways.

Protein type: Tumor suppressor; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: Golgi apparatus; perinuclear region of cytoplasm; cytoplasmic microtubule; cytoplasmic membrane-bound vesicle; postsynaptic density; cytoplasm; cell cortex; cytoplasmic vesicle; cytosol; beta-catenin destruction complex; nucleus; lateral plasma membrane

Molecular Function: protein C-terminus binding; identical protein binding; protein homodimerization activity; protein self-association; p53 binding; beta-catenin binding; protein kinase binding; GTPase activator activity; signal transducer activity; protein binding; receptor signaling complex scaffold activity; enzyme binding; ubiquitin protein ligase binding; protein complex scaffold; SMAD binding; receptor binding

Biological Process: cell death; protein polyubiquitination; negative regulation of Wnt receptor signaling pathway; apoptosis; embryonic eye morphogenesis; positive regulation of transcription, DNA-dependent; positive regulation of JNK cascade; negative regulation of protein metabolic process; dorsal/ventral axis specification; nucleocytoplasmic transport; Wnt receptor signaling pathway through beta-catenin; olfactory placode formation; activation of JNK activity; cytoplasmic microtubule organization and biogenesis; sensory perception of sound; positive regulation of ubiquitin-protein ligase activity; Wnt receptor signaling pathway involved in forebrain neuron fate commitment; protein catabolic process; protein homooligomerization; axial mesoderm formation; in utero embryonic development; positive regulation of transforming growth factor beta receptor signaling pathway; muscle cell development; negative regulation of fat cell differentiation; optic placode formation; positive regulation of peptidyl-serine phosphorylation; cellular protein complex assembly; positive regulation of protein ubiquitination; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; forebrain anterior/posterior pattern formation; activation of protein kinase activity; positive regulation of protein catabolic process; positive regulation of protein amino acid phosphorylation; determination of left/right symmetry; positive regulation of GTPase activity

Disease: Caudal Duplication Anomaly; Hepatocellular Carcinoma