Western Blot (WB)
(Western blot analysis of Rat Liver showing detection of ~93.5 kDa Axin-2 protein using Rabbit Anti-Axin-2 Polyclonal Antibody (SPC-749). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Axin-2 Polyclonal Antibody (SPC-749) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~93.5 kDa.)
Rabbit anti-Rat Axin-2 Polyclonal Antibody | anti-AXIN2 antibody
Axin-2 Antibody: HRP
Western Blot (WB)
(Western blot analysis of Rat Liver showing detection of ~93.5 kDa Axin-2 protein using Rabbit Anti-Axin-2 Polyclonal Antibody (SPC-749). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Axin-2 Polyclonal Antibody (SPC-749) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~93.5 kDa.)
Western Blot (WB)
(Western blot analysis of Rat Liver showing detection of ~93.5 kDa Axin-2 protein using Rabbit Anti-Axin-2 Polyclonal Antibody (SPC-749). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Axin-2 Polyclonal Antibody (SPC-749) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~93.5 kDa.)
NCBI and Uniprot Product Information
NCBI Description
The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair. [provided by RefSeq, Jul 2008]
Uniprot Description
Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B ().
Research Articles on AXIN2
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Product Notes
The AXIN2 axin2 (Catalog #AAA8000926) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Axin-2 Antibody: HRP reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Axin-2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000. Researchers should empirically determine the suitability of the AXIN2 axin2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Axin-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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