Rabbit anti-Human, Mouse AWAT2 Polyclonal Antibody | anti-AWAT2 antibody

AWAT2, CT (AWAT2, DC4, DGAT2L4, MFAT, WS, Acyl-CoA wax alcohol acyltransferase 2, Acyl-CoA retinol O-fatty-acyltransferase, Diacylglycerol O-acyltransferase 2-like protein 4, Diacylglycerol O-acyltransferase candidate 4, Long-chain-alcohol O-fatty-acyltra

Cat. Num. AAA6276990

• Gene Names: AWAT2; WS; DC4; ARAT; MFAT; DGAT2L4
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: AWAT2; Polyclonal Antibody; CT (AWAT2; DC4; DGAT2L4; MFAT; WS; Acyl-CoA wax alcohol acyltransferase 2; Acyl-CoA retinol O-fatty-acyltransferase; Diacylglycerol O-acyltransferase 2-like protein 4; Diacylglycerol O-acyltransferase candidate 4; Long-chain-alcohol O-fatty-acyltra; Long-chain-alcohol O-fatty-acyltransferase 2; Multifunctional O-acyltransferase; Wax synthase; anti-AWAT2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-AWAT2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: AWAT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 306-335 amino acids from the C-terminal region of human AWAT2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(AWAT2 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AWAT2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(AWAT2 Antibody (C-term) western blot analysis in mouse liver,spleen tissue lysates (35ug/lane).This demonstrates the AWAT2 antibody detected the AWAT2 protein (arrow).)

Product Categories/Family for anti-AWAT2 antibody

Antibodies; Enzymes; Acyltransferase

References

Holmes, R.S. Comp. Biochem. Physiol. Part D Genomics Proteomics 5(1):45-54(2010). Yen, C.L., et al. J. Lipid Res. 46(11):2388-2397(2005). Turkish, A.R., et al. J. Biol. Chem. 280(15):14755-14764(2005). Cheng, J.B., et al. J. Biol. Chem. 279(36):37798-37807(2004). Winter, A., et al. Cytogenet. Genome Res. 102 (1-4), 42-47 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 51317389
• NCBI GeneID: 158835
• NCBI Accession #: NP_001002254.1
• NCBI GenBank Nucleotide #: NM_001002254.1
• UniProt Accession #: Q6E213; Q6IEE3; Q6P437
• Molecular Weight: 38,094 Da
• NCBI Official Full Name: acyl-CoA wax alcohol acyltransferase 2
• NCBI Official Synonym Full Names: acyl-CoA wax alcohol acyltransferase 2
• NCBI Official Symbol: AWAT2
• NCBI Official Synonym Symbols: WS; DC4; ARAT; MFAT; DGAT2L4
• NCBI Protein Information: acyl-CoA wax alcohol acyltransferase 2
• UniProt Protein Name: Acyl-CoA wax alcohol acyltransferase 2
• Protein Family: Acyl-CoA wax alcohol acyltransferase
• UniProt Gene Name: AWAT2
• UniProt Synonym Gene Names: DC4; DGAT2L4; MFAT; WS; ARAT; Retinol O-fatty-acyltransferase; hDC4; hWS
• UniProt Entry Name: AWAT2_HUMAN

NCBI Description

This gene encodes an enzyme belonging to the diacylglycerol acyltransferase family. This enzyme produces wax esters by the esterification of long chain (or wax) alcohols with acyl-CoA-derived fatty acids. It functions in lipid metabolism in the skin, mostly in undifferentiated peripheral sebocytes. This enzyme may also have acyl-CoA:retinol acyltransferase activities, where it catalyzes the synthesis of diacylglycerols and retinyl esters. [provided by RefSeq, May 2010]

Uniprot Description

DGAT2L4: Acyltransferase that predominantly esterify long chain (wax) alcohols with acyl-CoA-derived fatty acids to produce wax esters. Wax esters are enriched in sebum, suggesting that it plays a central role in lipid metabolism in skin. Has no activity using decyl alcohol and significantly prefers the C16 and C18 alcohols. May also have 2-acylglycerol O-acyltransferase (MGAT) and acyl- CoA:retinol acyltransferase (ARAT) activities, to catalyze the synthesis of diacylglycerols and retinyl esters; however this activity is unclear in vivo. Belongs to the diacylglycerol acyltransferase family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Cofactor and Vitamin Metabolism - retinol; EC 2.3.1.75; EC 2.3.1.76; Lipid Metabolism - glycerolipid; Transferase

Chromosomal Location of Human Ortholog: Xq13.1

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: retinol O-fatty-acyltransferase activity; long-chain-alcohol O-fatty-acyltransferase activity

Biological Process: retinol metabolic process; wax biosynthetic process