Rabbit anti-Human AUP1 Polyclonal Antibody | anti-AUP1 antibody

AUP1, CT (AUP1, Ancient ubiquitous protein 1) (Azide free) (HRP)

Cat. Num. AAA6276847

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: AUP1; Polyclonal Antibody; CT (AUP1; Ancient ubiquitous protein 1) (Azide free) (HRP); Ancient ubiquitous protein 1; anti-AUP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-AUP1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: AUP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 439-468 amino acids from the C-terminal region of human AUP1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(AUP1 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the AUP1 antibody detected the AUP1 protein (arrow).)

Related Product Information for anti-AUP1 antibody

This gene encodes a protein that contains a domain with homology to the ancient conserved region of the archain 1 gene and a domain that may be involved in binding ubiquitin-conjugating enzymes. The protein encoded by this gene has been shown to bind to the conserved membrane-proximal sequence of the cytoplasmic tail of integrin alpha(IIb) subunits. These subunits play a crucial role in the integrin alpha(IIb)beta(3) inside-out signalling in platelets and megakaryocytes that leads to platelet aggregation and thrombus formation. This gene overlaps the gene for mitochondrial serine protease 25.

Product Categories/Family for anti-AUP1 antibody

Antibodies; Proteins

References

Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Kato, A., et al. J. Biol. Chem. 277(32):28934-28941(2002). Karpisheva, K.V., et al. Tsitologiia 44(9):846-851(2002). Karpisheva, K.V., et al. Tsitologiia 44(9):839-845(2002). Jang, W., et al. Genomics 36(2):366-368(1996).

NCBI and Uniprot Product Information

• NCBI GI #: 31712030
• NCBI GeneID: 550
• NCBI Accession #: NP_853553.1
• NCBI GenBank Nucleotide #: NM_181575.3
• UniProt Accession #: Q9Y679; Q68CX8
• Molecular Weight: 41,587 Da
• NCBI Official Full Name: ancient ubiquitous protein 1
• NCBI Official Synonym Full Names: ancient ubiquitous protein 1
• NCBI Official Symbol: AUP1
• NCBI Protein Information: ancient ubiquitous protein 1; OTTHUMP00000160424; OTTHUMP00000202724
• UniProt Protein Name: Ancient ubiquitous protein 1
• Protein Family: Ancient ubiquitous protein
• UniProt Gene Name: AUP1
• UniProt Entry Name: AUP1_HUMAN

NCBI Description

This gene encodes a protein that contains a domain with homology to the ancient conserved region of the archain 1 gene and a domain that may be involved in binding ubiquitin-conjugating enzymes. The protein encoded by this gene has been shown to bind to the conserved membrane-proximal sequence of the cytoplasmic tail of integrin alpha(IIb) subunits. These subunits play a crucial role in the integrin alpha(IIb)beta(3) inside-out signalling in platelets and megakaryocytes that leads to platelet aggregation and thrombus formation. This gene overlaps the gene for mitochondrial serine protease 25. [provided by RefSeq]