Rabbit AUP1 Polyclonal Antibody | anti-AUP1 antibody

AUP1 Polyclonal Antibody

Cat. Num. AAA2517433

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen affinity purification
• Synonyms: AUP1; Polyclonal Antibody; AUP1 Polyclonal Antibody; anti-AUP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1.9mg/mL (varies by lot)
• Sequence Length: 410

• Applicable Applications for anti-AUP1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human AUP1
• Buffer: PBS with 0.05% sodium azide, 50% glycerol, pH7.3
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

(Western Blot analysis of Hela and 293T cell using AUP1 Polyclonal Antibody at dilution of 1:500)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human liver cancer using AUP1 Polyclonal Antibody at dilution of 1:50)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human lung cancer using AUP1 Polyclonal Antibody at dilution of 1:50)

Related Product Information for anti-AUP1 antibody

This gene encodes a protein that contains a domain with homology to the ancient conserved region of the archain 1 gene and a domain that may be involved in binding ubiquitin-conjugating enzymes. The protein encoded by this gene has been shown to bind to the conserved membrane-proximal sequence of the cytoplasmic tail of integrin alpha(IIb) subunits. These subunits play a crucial role in the integrin alpha(IIb)beta(3) inside-out signalling in platelets and megakaryocytes that leads to platelet aggregation and thrombus formation. This gene overlaps the gene for mitochondrial serine protease 25.

Product Categories/Family for anti-AUP1 antibody

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 31712030
• NCBI GeneID: 550
• NCBI Accession #: NP_853553.1
• NCBI GenBank Nucleotide #: NM_181575.4
• Molecular Weight: 41,385 Da
• NCBI Official Full Name: ancient ubiquitous protein 1
• NCBI Official Synonym Full Names: ancient ubiquitous protein 1
• NCBI Official Symbol: AUP1
• NCBI Protein Information: ancient ubiquitous protein 1
• UniProt Protein Name: Ancient ubiquitous protein 1
• Protein Family: Ancient ubiquitous protein
• UniProt Gene Name: AUP1
• UniProt Entry Name: AUP1_HUMAN

NCBI Description

The protein encoded this gene is involved in several pathways including quality control of misfolded proteins in the endoplasmic reticulum and lipid droplet accumulation. Lipid droplets are organelles in the cytoplasm that store neutral lipids such as cholesterol esters and trigylycerides to prevent the overabundance of free cholesterol and fatty acids in cells, but also to act as storage for other metabolic processes, such as membrane biogenesis. Reduced expression of this gene results in reduced lipid droplet clustering, a function that is dependent on ubiquitination of the protein. This protein contains multiple domains including a hydrophobic N-terminal domain, an acetyltranferase domain, a ubiquitin-binding CUE domain, and a UBE2B2-binding domain (G2BR). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

Uniprot Description

AUP1: May play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. Belongs to the AUP1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; Membrane protein, integral; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: endoplasmic reticulum membrane; membrane; integral to membrane

Molecular Function: protein binding