Rabbit anti-Human ATRIP Polyclonal Antibody | anti-ATRIP antibody

ATRIP, Phosphorylated (pS239) (ATRIP, AGS1, ATR-interacting protein, ATM and Rad3-related-interacting protein) (MaxLight 650)

Cat. Num. AAA6276796

• Reactivity: Human
• Applications: Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ATRIP; Polyclonal Antibody; Phosphorylated (pS239) (ATRIP; AGS1; ATR-interacting protein; ATM and Rad3-related-interacting protein) (MaxLight 650); ATM and Rad3-related-interacting protein; anti-ATRIP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-ATRIP antibody: FLISA, Dot Blot (DB)
• Application Notes: FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Phospho-ATRIP-pS239 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide surrounding S239 of human ATRIP.
• Conjugate: MaxLight650
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-ATRIP antibody

The product of this gene is an essential component of the DNA damage checkpoint, and binds to single-stranded DNA coated with replication protein A that accumulates at sites of DNA damage. The encoded protein interacts with the ataxia telangiectasia and Rad3 related protein, a checkpoint kinase, resulting in accumulation of the kinase at intranuclear foci induced by DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-ATRIP antibody

Antibodies; DNA Replication; Repair

References

Bailey, S.D., et al. Diabetes Care (2010). In press: Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Vega, A., et al. Gynecol. Oncol. 112(1):210-214(2009). Myers, J.S., et al. Cancer Res. 67(14):6685-6690(2007). Venere, M., et al. Cancer Res. 67(13):6100-6105(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 18390344
• NCBI GeneID: 84126
• NCBI Accession #: NP_115542.2
• NCBI GenBank Nucleotide #: NM_032166.3
• UniProt Accession #: Q8WXE1; Q69YK9; Q8NHQ2; Q8WUG7; Q96CL3; Q9HA30; A8K6A3; A8K714; B2RCE7; B4DU92; B5MEB7
• Molecular Weight: 72,362 Da
• NCBI Official Full Name: ATR-interacting protein isoform 2
• NCBI Official Synonym Full Names: ATR interacting protein
• NCBI Official Symbol: ATRIP
• NCBI Protein Information: ATR-interacting protein
• UniProt Protein Name: ATR-interacting protein
• Protein Family: ATR-interacting protein
• UniProt Gene Name: ATRIP
• UniProt Synonym Gene Names: AGS1
• UniProt Entry Name: ATRIP_HUMAN

NCBI Description

This gene encodes an essential component of the DNA damage checkpoint. The encoded protein binds to single-stranded DNA coated with replication protein A. The protein also interacts with the ataxia telangiectasia and Rad3 related protein kinase, resulting in its accumulation at intranuclear foci induced by DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2012]

Uniprot Description

ATRIP: is required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein. Heterodimerizes with ATR. The heterodimer binds replication protein A (RPA), a protein complex that subsequently is recruited to single stranded DNA (ssDNA). Its EEXXXDDL motif is required for the interaction with catalytic subunit of DNA-PK and its recruitment to sites of DNA damage. e for this protein is either identical to or adjacent to that of ATRIP. Some of the mRNAs that encode ATRIP also encode TREX1 in another reading frame. Three alternatively spliced human isoforms have been reported.

Protein type: DNA repair, damage; DNA-binding; Cell cycle regulation

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding

Biological Process: DNA damage checkpoint; DNA repair; DNA replication