Rabbit anti-Mouse ATP8 Polyclonal Antibody | anti-ATP8 antibody

ATP8 Polyclonal Antibody

Cat. Num. AAA3001546

• Gene Names: mt-Atp8; ATP8
• Reactivity: Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ATP8; Polyclonal Antibody; ATP8 Polyclonal Antibody; ATP synthase protein 8; A6L; F-ATPase subunit 8; Mtatp8; Atp8; mt-Atp8; anti-ATP8 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ATP8 polyclonal antibody detects endogenous levels of ATP8 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 67

• Applicable Applications for anti-ATP8 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: A synthetic peptide corresponding to amino acids 46-60 of Mouse ATP8.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ATP8 polyclonal antibody at 1:1000 dilutionLane1:The heart tissue lysate of Mouse (38ug))

Related Product Information for anti-ATP8 antibody

Mitochondrial ATP synthase is composed of two multi-subunit complexes that utilize an inner membrane electrochemical gradient to catalyze the synthesis of ATP during oxidative phosphorylation. The two multi-subunit complexes are designated F1 and F0, the former of which comprises the soluble catalytic core and the latter of which comprises the membrane-spanning proton channel of ATP synthase. ATP8, also known as MT-ATP8, ATPASE8 or MTATP8, is a 68 amino acid single-pass membrane protein that localizes to mitochondria and functions as a minor subunit of the F0 complex. The gene encoding ATP8 maps to the mitochondrial chromosome and may be involved in the pathogenesis of multiple sclerosis.

NCBI and Uniprot Product Information

• NCBI GI #: 34538602
• NCBI GeneID: 17706
• NCBI Accession #: NP_904332.1
• NCBI GenBank Nucleotide #: NP_904332.1
• UniProt Accession #: P03930
• Molecular Weight: ~ 8kDa
• NCBI Official Full Name: ATP synthase F0 subunit 8 (mitochondrion)
• NCBI Official Synonym Full Names: ATP synthase 8, mitochondrial
• NCBI Official Symbol: mt-Atp8
• NCBI Official Synonym Symbols: ATP8
• NCBI Protein Information: ATP synthase F0 subunit 8
• UniProt Protein Name: ATP synthase protein 8
• Protein Family: ATP synthase protein
• UniProt Gene Name: Mtatp8
• UniProt Synonym Gene Names: Atp8; mt-Atp8
• UniProt Entry Name: ATP8_MOUSE

Uniprot Description

ATP8: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane. Belongs to the ATPase protein 8 family.

Protein type: Membrane protein, integral; Hydrolase; Mitochondrial; EC 3.6.3.14; Transporter

Cellular Component: mitochondrion; membrane; proton-transporting ATP synthase complex, coupling factor F(o); integral to membrane; mitochondrial proton-transporting ATP synthase complex

Molecular Function: hydrogen ion transmembrane transporter activity

Biological Process: ATP synthesis coupled proton transport; proton transport; transport; ATP biosynthetic process; ion transport