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Western Blot (WB) (Western blot analysis of 1) Hela Cell Lysate, 2) Mouse Heart Tissue Lysate, 3) Rat Heart Tissue Lysate using Claudin-2 (EM1403) Mouse mAb diluted at 1:2000.)

Mouse Claudin-2 Monoclonal Antibody | anti-CLDN2 antibody

Claudin-2 (8H11) Mouse mAb

Reactivity
Human, Rat, Mouse
Applications
Western Blot, Immunohistochemistry
Synonyms
Claudin-2; Monoclonal Antibody; Claudin-2 (8H11) Mouse mAb; anti-CLDN2 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Rat, Mouse
Clonality
Monoclonal
Isotype
IgG1
Form/Format
PBS with 0.02% sodium azide and 50% glycerol pH7.4.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CLDN2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:1,000-2,000
IHC: 1:100-1:200
Species
Human
Immunogen
Synthetic Peptide
Preparation and Storage
Store at -20 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of 1) Hela Cell Lysate, 2) Mouse Heart Tissue Lysate, 3) Rat Heart Tissue Lysate using Claudin-2 (EM1403) Mouse mAb diluted at 1:2000.)

Western Blot (WB) (Western blot analysis of 1) Hela Cell Lysate, 2) Mouse Heart Tissue Lysate, 3) Rat Heart Tissue Lysate using Claudin-2 (EM1403) Mouse mAb diluted at 1:2000.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human Kidney Carcinoma Tissue using Claudin-2 (EM1403)Mouse mAb diluted at 1:200.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human Kidney Carcinoma Tissue using Claudin-2 (EM1403)Mouse mAb diluted at 1:200.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human Endometrial Carcinoma Tissue using Claudin-2 (EM1403)Mouse mAb diluted at 1:200.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human Endometrial Carcinoma Tissue using Claudin-2 (EM1403)Mouse mAb diluted at 1:200.)
Related Product Information for anti-CLDN2 antibody
The claudin family is composed of 23 integral membrane proteins, and their expression, which varies among tissue types, may determine both the strength and properties of the epithelial barrier. Alteration in claudin protein expression pattern is associated with several types of cancer. Claudin-2 is expressed primarily in the proximal tubule of the normal mammalian kidney, where it regulates transepithelial ion (e.g., Na+, Cl-) reabsorption. Increased expression of Claudin-2 has been reported in some cancer cell lines, including A549 lung adenocarcinoma cells, where its nuclear distribution was positively associated with enhanced proliferation.
Product Categories/Family for anti-CLDN2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,549 Da
NCBI Official Full Name
claudin-2
NCBI Official Synonym Full Names
claudin 2
NCBI Official Symbol
CLDN2
NCBI Protein Information
claudin-2; SP82
UniProt Protein Name
Claudin-2
Protein Family
UniProt Gene Name
CLDN2
UniProt Entry Name
CLD2_HUMAN

NCBI Description

This gene product belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. This protein is expressed in the intestine. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene.[provided by RefSeq, Jan 2010]

Uniprot Description

Claudin-2: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Belongs to the claudin family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Cytoskeletal

Chromosomal Location of Human Ortholog: Xq22.3-q23

Cellular Component: tight junction; plasma membrane; integral to membrane

Molecular Function: identical protein binding; structural molecule activity

Biological Process: calcium-independent cell-cell adhesion

Research Articles on CLDN2

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Product Notes

The CLDN2 cldn2 (Catalog #AAA8808714) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Claudin-2 (8H11) Mouse mAb reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Claudin-2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:1,000-2,000 IHC: 1:100-1:200. Researchers should empirically determine the suitability of the CLDN2 cldn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Claudin-2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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