Rabbit ATP6V1E1 Polyclonal Antibody | anti-ATP6V0E1 antibody

ATP6V1E1 Antibody

Cat. Num. AAA9612269

• Gene Names: ATP6V0E1; M9.2; ATP6H; Vma21; Vma21p; ATP6V0E
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(88%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: ATP6V1E1; Polyclonal Antibody; ATP6V1E1 Antibody; anti-ATP6V0E1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(88%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ATP6V1E1 Antibody detects endogenous levels of total ATP6V1E1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 81

• Applicable Applications for anti-ATP6V0E1 antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human ATP6V1E1, corresponding to a region within N-terminal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Mouse spleen, using ATP6V1E1 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 25 kDa.)

Related Product Information for anti-ATP6V0E1 antibody

Function: Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

Subcellular Location: Membrane>Multi-pass membrane protein.

Tissue Specificity: Ubiquitous.

Subunit Structure: Composed of at least 10 subunits.

Similarity: Belongs to the V-ATPase e1/e2 subunit family.

NCBI and Uniprot Product Information

• NCBI GI #: 4502319
• NCBI GeneID: 8992
• NCBI Accession #: NP_003936.1
• NCBI GenBank Nucleotide #: NP_003936.1
• UniProt Accession #: O15342
• Molecular Weight: Observed Molecular Weight: (Observed)9kD, 25kD.; Predicted Molecular Weight: (Calculated)9kDa.
• NCBI Official Full Name: V-type proton ATPase subunit e 1
• NCBI Official Synonym Full Names: ATPase H+ transporting V0 subunit e1
• NCBI Official Symbol: ATP6V0E1
• NCBI Official Synonym Symbols: M9.2; ATP6H; Vma21; Vma21p; ATP6V0E
• NCBI Protein Information: V-type proton ATPase subunit e 1
• UniProt Protein Name: V-type proton ATPase subunit e 1
• Protein Family: V-type proton ATPase
• UniProt Gene Name: ATP6V0E1
• UniProt Synonym Gene Names: ATP6H; ATP6V0E; V-ATPase subunit e 1
• UniProt Entry Name: VA0E1_HUMAN

NCBI Description

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is possibly part of the V0 subunit. Since two nontranscribed pseudogenes have been found in dog, it is possible that the localization to chromosome 2 for this gene by radiation hybrid mapping is representing a pseudogene. Genomic mapping puts the chromosomal location on 5q35.3. [provided by RefSeq, Jul 2008]