Bcl2 Associated Athanogene 3 Recombinant Protein | BAG3 recombinant protein

Recombinant Bcl2 Associated Athanogene 3 (BAG3)

Cat. Num. AAA2122947

• Gene Names: BAG3; BIS; MFM6; BAG-3; CAIR-1
• Applications: SDS-Page, Western Blot
• Purity: >90%
• Synonyms: Bcl2 Associated Athanogene 3; Recombinant Bcl2 Associated Athanogene 3 (BAG3); BIS; CAIR1; BAG Family Molecular Chaperone Regulator 3; Bcl-2-binding protein Bis; Docking protein CAIR-1; BAG3 recombinant protein

• Host: E Coli
• Purity/Purification: >90%
• Form/Format: Freeze-dried powder; 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Sequence Positions: Gly421~Ala498
• Sequence Length: 575

• Applicable Applications for BAG3 recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Source: Prokaryotic expression
• Species: Human
• Tag: N-terminal His Tag
• Subcellular Location: Nucleus, Cytoplasm
• Isoelectric Point: 5.2
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.; Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

(Figure. SDS-PAGE)

Product Categories/Family for BAG3 recombinant protein

Apoptosis; Neuro science

NCBI and Uniprot Product Information

• NCBI GI #: 14043024
• NCBI GeneID: 9531
• NCBI Accession #: NP_004272.2
• NCBI GenBank Nucleotide #: NP_004272.2
• UniProt Accession #: O95817
• Molecular Weight: Predicted Molecular Mass: 12.6kDa; Accurate Molecular Mass: 15kDa
• NCBI Official Full Name: BAG family molecular chaperone regulator 3
• NCBI Official Synonym Full Names: BAG cochaperone 3
• NCBI Official Symbol: BAG3
• NCBI Official Synonym Symbols: BIS; MFM6; BAG-3; CAIR-1
• NCBI Protein Information: BAG family molecular chaperone regulator 3
• UniProt Protein Name: BAG family molecular chaperone regulator 3
• Protein Family: BAG family molecular chaperone regulator
• UniProt Gene Name: BAG3
• UniProt Synonym Gene Names: BIS; BAG-3
• UniProt Entry Name: BAG3_HUMAN

NCBI Description

BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008]

Uniprot Description

BAG3: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity. Defects in BAG3 are the cause of myopathy myofibrillar type 6 (MFM6). A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material. Defects in BAG3 are the cause of cardiomyopathy dilated type 1HH (CMD1HH). CMD1HH is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 10q25.2-q26.2

Cellular Component: neuron projection; cytoplasm; plasma membrane; cytosol; Z disc

Molecular Function: protein binding; chaperone binding; protein complex binding

Biological Process: induction of apoptosis via death domain receptors; protein folding; protein stabilization; spinal cord development; brain development; negative regulation of apoptosis

Disease: Myopathy, Myofibrillar, 6; Cardiomyopathy, Dilated, 1hh