Rabbit ATP6V0D1 Polyclonal Antibody | anti-ATP6V0D1 antibody

ATP6V0D1 Polyclonal Antibody

Cat. Num. AAA3006290

• Gene Names: ATP6V0D1; P39; VATX; VMA6; ATP6D; ATP6DV; VPATPD
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ATP6V0D1; Polyclonal Antibody; ATP6V0D1 Polyclonal Antibody; V-type proton ATPase subunit d 1; V-ATPase subunit d 1; 32 kDa accessory protein; V-ATPase 40 kDa accessory protein; V-ATPase AC39 subunit; Short name=p39; Vacuolar proton pump subunit d 1; ATP6D; VPATPD; V-ATPase D1; anti-ATP6V0D1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ATP6V0D1 polyclonal antibody detects endogenous levels of ATP6V0D1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 351

• Applicable Applications for anti-ATP6V0D1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 280-325 of Human ATP6V0D1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ATP6V0D1 polyclonal antibody at 1:500 dilutionLane1:HEK293T cell lysateLane2:sp2/0 cell lysateLane3:H9C2 cell lysate)

Related Product Information for anti-ATP6V0D1 antibody

Vacuolar-type H+-ATPase (V-ATPase) is a multisubunit enzyme responsible for the acidification of eukaryotic intracellular organelles. V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process, thereby synthesizing ATP. A peripheral V1 domain, which is responsible for ATP hydrolysis, and an integral V0 domain, which is responsible for proton translocation, comprise the V-ATPase complex. Nine subunits (A-H) make up the V1 domain and five subunits (A, D, C, C' and C") make up the V0 domain. V-ATPase D1 (ATPase, H+ transporting, lysosomal, V0 subunit D1), also known as ATP6V0D1, P39, VATX, VMA6, ATP6D or VPATPD, is the D subunit of the V0 domain. Expressed ubiquitously, V-ATPase D1 acts in concert with other V0 subunits to catalytically acidify a variety of intracellular compartments, thereby synthesizing ATP to be used for vacuolar transport.

NCBI and Uniprot Product Information

• NCBI GI #: 47606646
• NCBI GeneID: 9114
• UniProt Accession #: P61421
• Molecular Weight: ~ 40kDa
• NCBI Official Full Name: V-type proton ATPase subunit d 1
• NCBI Official Synonym Full Names: ATPase H+ transporting V0 subunit d1
• NCBI Official Symbol: ATP6V0D1
• NCBI Official Synonym Symbols: P39; VATX; VMA6; ATP6D; ATP6DV; VPATPD
• NCBI Protein Information: V-type proton ATPase subunit d 1
• UniProt Protein Name: V-type proton ATPase subunit d 1
• Protein Family: V-type proton ATPase
• UniProt Gene Name: ATP6V0D1
• UniProt Synonym Gene Names: ATP6D; VPATPD; V-ATPase subunit d 1; p39
• UniProt Entry Name: VA0D1_HUMAN

NCBI Description

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is known as the D subunit and is found ubiquitously. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP6V0D1: Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis. Belongs to the V-ATPase V0D/AC39 subunit family.

Protein type: Hydrolase; Vesicle; EC 3.6.3.14; Energy Metabolism - oxidative phosphorylation

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: centrosome; phagocytic vesicle membrane; synaptic vesicle; membrane; lysosomal membrane; apical plasma membrane; early endosome; endosome membrane; vacuolar proton-transporting V-type ATPase complex; nerve terminal

Molecular Function: hydrogen-exporting ATPase activity, phosphorylative mechanism; protein binding; protein complex binding

Biological Process: interaction with host; proton transport; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; unfolded protein response; cellular iron ion homeostasis; ATP hydrolysis coupled proton transport; insulin receptor signaling pathway; transferrin transport; cilium biogenesis; brain development; transmembrane transport