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Western Blot (WB) (Western blot analysis using IL2RA mAb against human IL2RA recombinant protein. (Expected MW is 37.5 kDa))

Mouse IL2RA Monoclonal Antibody | anti-IL2RA antibody

Anti-IL2RA Mouse mAb

Gene Names
IL2RA; p55; CD25; IL2R; IMD41; TCGFR; IDDM10
Reactivity
Human, Mouse, Monkey, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Synonyms
IL2RA; Monoclonal Antibody; Anti-IL2RA Mouse mAb; CD25; IL2R; TCGFR; IDDM10; anti-IL2RA antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse, Monkey, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1B5D12
Form/Format
Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer
Sequence Length
176
Applicable Applications for anti-IL2RA antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Immunogen
Purified recombinant fragment of human IL2RA (AA: 34-139) expressed in E Coli.

Western Blot (WB)

(Western blot analysis using IL2RA mAb against human IL2RA recombinant protein. (Expected MW is 37.5 kDa))

Western Blot (WB) (Western blot analysis using IL2RA mAb against human IL2RA recombinant protein. (Expected MW is 37.5 kDa))

Western Blot (WB)

(Western blot analysis using IL2RA mAb against HEK293 (1) and IL2RA (AA)

Western Blot (WB) (Western blot analysis using IL2RA mAb against HEK293 (1) and IL2RA (AA)

Western Blot (WB)

(Western blot analysis using IL2RA mouse mAb against Hela (1), MOLT4 (2), HEK293 (3), A549 (4), Jurkat (5), K562 (6), Cos7 (7), PC-12 (8) and NIH/3T3 (9) cell lysate.)

Western Blot (WB) (Western blot analysis using IL2RA mouse mAb against Hela (1), MOLT4 (2), HEK293 (3), A549 (4), Jurkat (5), K562 (6), Cos7 (7), PC-12 (8) and NIH/3T3 (9) cell lysate.)

Immunofluorescence (IF)

(Immunofluorescence analysis of Hela cells using IL2RA mouse mAb (green). Blue)

Immunofluorescence (IF) (Immunofluorescence analysis of Hela cells using IL2RA mouse mAb (green). Blue)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using IL2RA mouse mAb with DAB staining.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using IL2RA mouse mAb with DAB staining.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using IL2RA mouse mAb with DAB staining.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using IL2RA mouse mAb with DAB staining.)
Related Product Information for anti-IL2RA antibody
Entrez Summary: The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30.8
NCBI Official Full Name
interleukin-2 receptor subunit alpha isoform 3
NCBI Official Synonym Full Names
interleukin 2 receptor subunit alpha
NCBI Official Symbol
IL2RA
NCBI Official Synonym Symbols
p55; CD25; IL2R; IMD41; TCGFR; IDDM10
NCBI Protein Information
interleukin-2 receptor subunit alpha
UniProt Protein Name
Interleukin-2 receptor subunit alpha
Protein Family
UniProt Gene Name
IL2RA
UniProt Synonym Gene Names
IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA

NCBI Description

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]

Uniprot Description

IL2RA: Receptor for interleukin-2. Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 10p15.1

Cellular Component: plasma membrane

Molecular Function: interleukin-2 binding; Ras guanyl-nucleotide exchange factor activity

Biological Process: apoptosis; cell proliferation; cell surface receptor linked signal transduction; immune response; inflammatory response; MAPKKK cascade; regulation of T cell tolerance induction

Disease: Diabetes Mellitus, Insulin-dependent, 10; Interleukin 2 Receptor, Alpha, Deficiency Of

Research Articles on IL2RA

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Product Notes

The IL2RA il2ra (Catalog #AAA476433) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-IL2RA Mouse mAb reacts with Human, Mouse, Monkey, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's IL2RA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Researchers should empirically determine the suitability of the IL2RA il2ra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL2RA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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