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Western Blot (WB) (Western blot analysis of ATP11A in K562 cell tissue lysate with ATP11A antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

Rabbit ATP11A Polyclonal Antibody | anti-ATP11A antibody

ATP11A Antibody

Gene Names
ATP11A; ATPIH; ATPIS
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
ATP11A Antibody is affinity chromatography purified via peptide column.
Synonyms
ATP11A; Polyclonal Antibody; ATP11A Antibody; ATPIH; ATPIS; KIAA1021; Probable phospholipid-transporting ATPase IH; ATPase IS; ATPase; class VI; type 11A; anti-ATP11A antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
ATP11A Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
1191
Applicable Applications for anti-ATP11A antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
ATP11A antibody can be used for detection of ATP11A by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
ATP11A antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human ATP11A.
Buffer
ATP11A Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
ATP11A antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of ATP11A in K562 cell tissue lysate with ATP11A antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

Western Blot (WB) (Western blot analysis of ATP11A in K562 cell tissue lysate with ATP11A antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

Immunocytochemistry (IHC)

(Immunocytochemistry of ATP11A in K562 cells with ATP11A antibody at 10 μg/mL.)

Immunocytochemistry (IHC) (Immunocytochemistry of ATP11A in K562 cells with ATP11A antibody at 10 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of ATP11A in K562 cells with ATP11A antibody at 20 μg/mL.)

Immunofluorescence (IF) (Immunofluorescence of ATP11A in K562 cells with ATP11A antibody at 20 μg/mL.)
Related Product Information for anti-ATP11A antibody
ATP11A Antibody: ATP11A is a widely expressed integral membrane ATPase. ATP11A is probably phosphorylated in its intermediate state and likely drives the transport of ions such as calcium or other molecules across membranes. While the exact molecule ATP11A transports is unknown, increased expression of ATP11A mRNA has been observed in murine leukemia cells made resistant to anti-cancer drugs such as farnesyltransferase inhibitors (FTIs). Furthermore, overexpression of ATP11A provided protection against the FTI SCH66336 while knockdown of ATP11A via siRNA made cells more sensitive to this drug. Other reports suggest that elevated levels of ATP11A mRNA may also be a predictive marker of metastasis in colorectal cancer patients.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
129,756 Da
NCBI Official Full Name
probable phospholipid-transporting ATPase IH isoform b
NCBI Official Synonym Full Names
ATPase, class VI, type 11A
NCBI Official Symbol
ATP11A
NCBI Official Synonym Symbols
ATPIH; ATPIS
NCBI Protein Information
probable phospholipid-transporting ATPase IH; phospholipid-translocating ATPase; potential phospholipid-transporting ATPase IH; P4-ATPase flippase complex alpha subunit ATP11A
UniProt Protein Name
Probable phospholipid-transporting ATPase IH
UniProt Gene Name
ATP11A
UniProt Synonym Gene Names
ATPIH; ATPIS; KIAA1021
UniProt Entry Name
AT11A_HUMAN

NCBI Description

The protein encoded by this gene is an integral membrane ATPase. The encoded protein is probably phosphorylated in its intermediate state and likely drives the transport of ions such as calcium across membranes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP11A: is an integral membrane ATPase. The encoded protein is probably phosphorylated in its intermediate state and likely drives the transport of ions such as calcium across membranes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Protein type: Membrane protein, multi-pass; Hydrolase; EC 3.6.3.1; Transporter, ion channel; Transporter; Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: recycling endosome; membrane; intracellular membrane-bound organelle; lysosomal membrane; endoplasmic reticulum; early endosome; plasma membrane; integral to membrane

Molecular Function: phospholipid-translocating ATPase activity; protein binding; magnesium ion binding; ATP binding

Biological Process: phospholipid translocation; metabolic process

Research Articles on ATP11A

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Product Notes

The ATP11A atp11a (Catalog #AAA151310) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATP11A Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATP11A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). ATP11A antibody can be used for detection of ATP11A by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the ATP11A atp11a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATP11A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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