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Western Blot (WB) (Western blot analysis of Rat Brain cell lysates showing detection of 101 kDa ATG9B protein using Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Primary Antibody: Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 101 kDa.)

Rabbit anti-Human; Mouse ATG9B Polyclonal Antibody | anti-ATG9B antibody

ATG9B Antibody: RPE

Gene Names
ATG9B; SONE; APG9L2; NOS3AS
Reactivity
Human; Mouse
Applications
Western Blot, Immunocytochemistry, Immunofluorescence
Purity
Peptide Affinity Purified
Synonyms
ATG9B; Polyclonal Antibody; ATG9B Antibody: RPE; Rabbit Anti-Human ATG9B Polyclonal; APG9-like 2 Antibody; APG9L2 Antibody; Nitric oxide synthase 3-overlapping antisense gene protein Antibody; Protein sONE Antibody; NOS3AS Antibody; ATG9B Antibody; Autophagy-related protein 9B Antibody; anti-ATG9B antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human; Mouse
Clonality
Polyclonal
Specificity
Detects ~100 kDa.
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (varies by lot)
Sequence Length
924
Applicable Applications for anti-ATG9B antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
WB: 1:1000
ICC/IF: 1:100
Target
ATG9B
Immunogen
Synthetic peptide from the N-terminal of Human ATG9B (aa. 110-121)
Immunogen Species
Human
Cellular Localization
Cytoplasmic Vesicle; Autophagosome Membrane; Multi-Pass Membrane Protein
Conjugate
RPE
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide
Tissue Specificity
Highly expressed in placenta (trophoblast cells) and pituitary gland. Not expressed in vascular endothelial.
Preparation and Storage
Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Rat Brain cell lysates showing detection of 101 kDa ATG9B protein using Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Primary Antibody: Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 101 kDa.)

Western Blot (WB) (Western blot analysis of Rat Brain cell lysates showing detection of 101 kDa ATG9B protein using Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Primary Antibody: Rabbit Anti-ATG9B Polyclonal Antibody (SPC-646) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 101 kDa.)
Related Product Information for anti-ATG9B antibody
ATG9B is involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Highly expressed in the placenta and the pituitary gland.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36,989 Da
NCBI Official Full Name
autophagy-related protein 9B
NCBI Official Synonym Full Names
autophagy related 9B
NCBI Official Symbol
ATG9B
NCBI Official Synonym Symbols
SONE; APG9L2; NOS3AS
NCBI Protein Information
autophagy-related protein 9B
UniProt Protein Name
Autophagy-related protein 9B
Protein Family
UniProt Gene Name
ATG9B
UniProt Synonym Gene Names
APG9L2; NOS3AS; Protein sONE

NCBI Description

This gene functions in the regulation of autophagy, a lysosomal degradation pathway. This gene also functions as an antisense transcript in the posttranscriptional regulation of the endothelial nitric oxide synthase 3 gene, which has 3' overlap with this gene on the opposite strand. Mutations in this gene and disruption of the autophagy process have been associated with multiple cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2012]

Uniprot Description

Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle ().

Research Articles on ATG9B

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Product Notes

The ATG9B atg9b (Catalog #AAA809776) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATG9B Antibody: RPE reacts with Human; Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ATG9B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:1000 ICC/IF: 1:100. Researchers should empirically determine the suitability of the ATG9B atg9b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATG9B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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