Testing Data
Rabbit ATG9B Polyclonal Antibody | anti-ATG9B antibody
ATG9B Antibody (CT)
Gene Names
ATG9B; SONE; APG9L2; NOS3AS
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, ELISA
Purity
Affinity chromatography purified via peptide column.
Synonyms
ATG9B; Polyclonal Antibody; ATG9B Antibody (CT); Autophagy-related protein 9B; APG9L2; APG9-like 2; Nitric oxide synthase 3-overlapping antisense gene protein; NOS3AS; anti-ATG9B antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG1
Purity/Purification
Affinity chromatography purified via peptide column.
Form/Format
100 ug (1 mg/ml) in 1X PBS containing 0.02% sodium azide.
Appearance: Colorless liquid
Appearance: Colorless liquid
Concentration
1 mg/ml (varies by lot)
Sequence Length
924
Applicable Applications for anti-ATG9B antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
Western Blot: 1 - 2 mug/ml, Immunocytochemistry: 10 mug/ml, Immunoflorescence : 20 mug/ml, ELISA. However, the optimal conditions should be determined individually.
Immunogen
ATG9B antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ATG9B.
Positive Control
Western Blot: HeLa cell lysateImmunocytochemistry: HeLa cellsImmunoflorescence: HeLa cells
Handling
The antibody solution should be gently mixed before use.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Testing Data
Testing Data
Related Product Information for anti-ATG9B antibody
Background: Autophagy, the process of bulk degradation of cellular proteins thromugh an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) thromugh phosphorylation of autophagy protein APG1. ATG9B plays a role in autophagy and it's highly expressed in placenta and pituitary gland.
Product Categories/Family for anti-ATG9B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,989 Da
NCBI Official Full Name
autophagy-related protein 9B
NCBI Official Synonym Full Names
autophagy related 9B
NCBI Official Symbol
ATG9B
NCBI Official Synonym Symbols
SONE; APG9L2; NOS3AS
NCBI Protein Information
autophagy-related protein 9B
UniProt Protein Name
Autophagy-related protein 9B
Protein Family
UniProt Gene Name
ATG9B
UniProt Synonym Gene Names
APG9L2; NOS3AS; Protein sONE
UniProt Entry Name
ATG9B_HUMAN
NCBI Description
This gene functions in the regulation of autophagy, a lysosomal degradation pathway. This gene also functions as an antisense transcript in the posttranscriptional regulation of the endothelial nitric oxide synthase 3 gene, which has 3' overlap with this gene on the opposite strand. Mutations in this gene and disruption of the autophagy process have been associated with multiple cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2012]
Uniprot Description
ATG9B: Plays a role in autophagy. Belongs to the ATG9 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; Vesicle; Autophagy
Chromosomal Location of Human Ortholog: 7q36.1
Cellular Component: integral to membrane; cytoplasmic vesicle
Biological Process: autophagic vacuole formation
Research Articles on ATG9B
Similar Products
Product Notes
The ATG9B atg9b (Catalog #AAA840653) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATG9B Antibody (CT) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATG9B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA). Western Blot: 1 - 2 mug/ml, Immunocytochemistry: 10 mug/ml, Immunoflorescence : 20 mug/ml, ELISA. However, the optimal conditions should be determined individually. Researchers should empirically determine the suitability of the ATG9B atg9b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATG9B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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