Rabbit ATG7 Polyclonal Antibody | anti-ATG7 antibody

Anti-ATG7 Antibody

Cat. Num. AAA1752925

• Gene Names: ATG7; GSA7; APG7L; APG7-LIKE
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Immunogen affinity purified.
• Synonyms: ATG7; Polyclonal Antibody; Anti-ATG7 Antibody; Ubiquitin-like modifier-activating enzyme ATG7; ATG12-activating enzyme E1 ATG7; Autophagy-related protein 7; APG7-like; hAGP7; Ubiquitin-activating enzyme E1-like protein; APG7L; anti-ATG7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 676

• Applicable Applications for anti-ATG7 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS)
• Application Notes: WB: 0.1-0.25ug/ml (Human, Rat); IHC-P (Embedded Section): 0.5-1ug/ml (Human, Mouse); FC/FACS: 1-3ug/1x106 cells (Human, Mouse); Tested Species: In-house tested species with positive results.
• Immunogen: A synthetic peptide corresponding to a sequence of human ATG7(HELTQKKLNEYRLDEAPKDIK).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Related Product Information for anti-ATG7 antibody

Description: Rabbit IgG polyclonal antibody for ATG7 detection. Tested with WB, IHC-P, FCM in Human; Mouse; Rat.

Background: Autophagy related 7 is a protein in humans encoded by ATG7 gene. It is mapped to 3p25.3. This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants.

References

1. Komatsu, M., Wang, Q. J., Holstein, G. R., Friedrich, V. L., Jr., Iwata, J., Kominami, E., Chait, B. T., Tanaka, K., Yue, Z. Essential role for autophagy protein Atg7 in the maintenance of axonal homeostasis and the prevention of axonal degeneration. Proc. Nat. Acad. Sci. 104: 14489-14494, 2007.
2. Lee, I. H., Kawai, Y., Fergusson, M. M., Rovira, I. I., Bishop, A. J. R., Motoyama, N., Cao, L., Finkel, T. Atg7 modulates p53 activity to regulate cell cycle and survival during metabolic stress. Science 336: 225-228, 2012. Note: Erratum: Science 337: 910 only, 2012. Erratum: Science 341: 457 only, 2013.
3. Nishida, Y., Arakawa, S., Fujitani, K., Yamaguchi, H., Mizuta, T., Kanaseki, T., Komatsu, M., Otsu, K., Tsujimoto, Y., Shimizu, S. Discovery of Atg5/Atg7-independent alternative macroautophagy. Nature 461: 654-658, 2009. Note: Erratum: Nature 533: 130 only, 2016.

NCBI and Uniprot Product Information

• NCBI GI #: 222144227
• NCBI GeneID: 10533
• NCBI Accession #: NP_001129503.2
• NCBI GenBank Nucleotide #: NP_001129503.2
• UniProt Accession #: O95352
• NCBI Official Full Name: ubiquitin-like modifier-activating enzyme ATG7 isoform b
• NCBI Official Synonym Full Names: autophagy related 7
• NCBI Official Symbol: ATG7
• NCBI Official Synonym Symbols: GSA7; APG7L; APG7-LIKE
• NCBI Protein Information: ubiquitin-like modifier-activating enzyme ATG7
• UniProt Protein Name: Ubiquitin-like modifier-activating enzyme ATG7
• Protein Family: Ubiquitin-like modifier-activating enzyme
• UniProt Gene Name: ATG7
• UniProt Synonym Gene Names: APG7L; APG7-like; hAGP7
• UniProt Entry Name: ATG7_HUMAN

NCBI Description

This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]