Rabbit anti-Human ATG5-ATG12 COMPLEX Polyclonal Antibody | anti-ATG5-ATG12 antibody

RABBIT ANTI ATG5-ATG12 COMPLEX (C-TERMINAL)

Cat. Num. AAA224276

• Gene Names: ATG12; APG12; FBR93; APG12L; HAPG12
• Reactivity: Human
• Applications: Western Blot
• Synonyms: ATG5-ATG12 COMPLEX; Polyclonal Antibody; RABBIT ANTI ATG5-ATG12 COMPLEX (C-TERMINAL); anti-ATG5-ATG12 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item specifically recognises an epitope within the C-Terminal (CT) region of the Atg5-Atg12 complex, which plays a key role in the regulation of autophagy, and is emerging as an important factor in the innate immune response to viruses. ; ; Studies have shown that the Atg5-Atg12 complex directly interacts with RIG-1 (retinoic acid-inducible gene 1) and the adaptor molecule IPS-1 (IFN-beta promoter stimulator 1), through caspase recruitment domains (CARDs), thereby acting as a negative regulator of the type I IFN production pathway, and as a contributor to RNA viral replication within the host cells.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 140

• Applicable Applications for anti-ATG5-ATG12 antibody: Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 56kDa in mouse liver and human U87 cell lysates.; Western Blotting: Minimum Dilution: 1.0; Maximum Dilution: 2.0ug/ml
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
• Immunogen: KLH conjugated synthetic peptide corresponding to amino acids 262-275 of mouse Atg5. The corresponding sequence differs by one amino acid in human.; Buffer Solution
• Antiserum Preparation: Antiserum to mouse Atg5 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-ATG5-ATG12 antibody

MBS224276 specifically recognises an epitope within the C-Terminal (CT) region of the Atg5-Atg12 complex, which plays a key role in the regulation of autophagy, and is emerging as an important factor in the innate immune response to viruses. Studies have shown that the Atg5-Atg12 complex directly interacts with RIG-1 (retinoic acid -inducible gene 1) and the adaptor molecule IPS-1 (IFN-beta promoter stimulator 1), through caspase recruitment domains (CARDs), thereby acting as a negative regulator of the type I IFN production pathway, and as a contributor to RNA viral replication within the host cells.

NCBI and Uniprot Product Information

• NCBI GI #: 290560746
• NCBI GeneID: 9140
• NCBI Accession #: NP_004698.3
• NCBI GenBank Nucleotide #: NM_004707.3
• Molecular Weight: 7,746 Da
• NCBI Official Full Name: ubiquitin-like protein ATG12 isoform 1
• NCBI Official Synonym Full Names: autophagy related 12
• NCBI Official Symbol: ATG12
• NCBI Official Synonym Symbols: APG12; FBR93; APG12L; HAPG12
• NCBI Protein Information: ubiquitin-like protein ATG12; ATG12 autophagy related 12 homolog; Apg12 (autophagy, yeast) homolog
• UniProt Protein Name: Ubiquitin-like protein ATG12
• UniProt Gene Name: ATG12
• UniProt Synonym Gene Names: APG12; APG12L; APG12-like
• UniProt Entry Name: ATG12_HUMAN

NCBI Description

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008]

Uniprot Description

ATG12: Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5. Ubiquitous. Belongs to the ATG12 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin-like modifier; Autophagy

Chromosomal Location of Human Ortholog: 5q21-q22

Cellular Component: pre-autophagosomal structure membrane

Molecular Function: protein binding; APG8 conjugating enzyme activity

Biological Process: mitochondrion degradation; innate immune response; C-terminal protein lipidation; negative regulation of interferon type I production; cellular response to nitrogen starvation; autophagic vacuole formation