Rabbit anti-Human, Mouse CASP6 Polyclonal Antibody | anti-CASP6 antibody

CASP6, CT (Caspase-6, CASP-6, Apoptotic Protease Mch-2, MCH2) (FITC)

Cat. Num. AAA6467540

• Gene Names: CASP6; MCH2
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: CASP6; Polyclonal Antibody; CT (Caspase-6; CASP-6; Apoptotic Protease Mch-2; MCH2) (FITC); anti-CASP6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CASP6. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CASP6 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human CASP6 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-CASP6 Antibody (C-term) in mouse liver tissue lysates (35ug/lane). CASP6 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CASP6 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-CASP6 antibody

Antibodies; Apoptosis; Caspase

References

1. Schmeck, B., et al., Infect. Immun. 72(9):4940-4947 (2004). 2. Mendez, E., et al., J. Virol. 78(16):8601-8608 (2004). 3. MacLachlan, T.K., et al., Proc. Natl. Acad. Sci. USA 99(14):9492-9497 (2002). 4. Sordet, O., et al., Leukemia 16(8):1569-1570 (2002). 5. LeBlanc, A., et al., J. Biol. Chem. 274(33):23426-23436 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 14916483
• NCBI GeneID: 839
• NCBI Accession #: NP_001217
• NCBI GenBank Nucleotide #: NM_001226.3
• UniProt Accession #: P55212; Q9BQE7
• Molecular Weight: 22,574 Da
• NCBI Official Full Name: caspase-6 isoform alpha preproprotein
• NCBI Official Synonym Full Names: caspase 6, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP6
• NCBI Official Synonym Symbols: MCH2
• NCBI Protein Information: caspase-6; apoptotic protease MCH-2; caspase 6, apoptosis-related cysteine protease
• UniProt Protein Name: Caspase-6
• Protein Family: Caspase
• UniProt Gene Name: CASP6
• UniProt Synonym Gene Names: MCH2; CASP-6
• UniProt Entry Name: CASP6_HUMAN

NCBI Description

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in two transcript variants that encode different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

CASP6: Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 11 kDa (p11) subunit. Interacts with BIRC6/bruce. Activation is suppressed by phosphorylation at Ser-257. Belongs to the peptidase C14A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.59; Protease; Apoptosis

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function: identical protein binding; protein binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: epithelial cell differentiation; apoptosis; proteolysis; cell structure disassembly during apoptosis