ACADSB recombinant protein

ACADSB, 34-432aa, Human, His tag, E Coli

Cat. Num. AAA204623

• Gene Names: ACADSB; ACAD7; SBCAD; 2-MEBCAD
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: ACADSB; 34-432aa; Human; His tag; E Coli; Short/branched chain specific acyl-CoA dehydrogenase mitochondrial; Short/branched chain specific acyl-CoA dehydrogenase; mitochondrial; 2-MEBCAD; ACAD7; SBCAD; ACADSB recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMKSSQS EALLNITNNG IHFAPLQTFT DEEMMIKSSV KKFAQEQIAP LVSTMDENSK MEKSVIQGLF QQGLMGIEVD PEYGGTGASF LSTVLVIEEL AKVDASVAVF CEIQNTLINT LIRKHGTEEQ KATYLPQLTT EKVGSFCLSE AGAGSDSFAL KTRADKEGDY YVLNGSKMWI SSAEHAGLFL VMANVDPTIG YKGITSFLVD RDTPGLHIGK PENKLGLRAS STCPLTFENV KVPEANILGQ IGHGYKYAIG SLNEGRIGIA AQMLGLAQGC FDYTIPYIKE RIQFGKRLFD FQGLQHQVAH VATQLEAARL LTYNAARLLE AGKPFIKEAS MAKYYASEIA GQTTSKCIEW MGGVGYTKDY PVEKYFRDAK IGTIYEGASN IQLNTIAKHI DAEY
• Sequence Length: 432

• Applicable Applications for ACADSB recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for ACADSB recombinant protein

Short/branched chain specific acyl-CoA dehydrogenase (ACADSB), is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. ACADSB catalyzes the degradation of L-isoleucine and has the highest affinity for (s)-2-methylbutyryl-CoA, isobutyryl-CoA and 2-methylhexanoyl-CoA as substrates. Defects in ACADSB are the cause of short/branched-chain acyl-CoA dehydrogenase deficiency (SBCADD), an autosomal recessive disorder characterized by an increase of 2-methylbutyrylglycine and 2-methylbutyrylcarnitine in blood and urine. Recombinant human ACADSB protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for ACADSB recombinant protein

Signal Transduction

References

Arden K C., et al. (1995) Genomics. 25: 743-745.; Korman S H., et al. (2005) Clin Chem. 51: 610-617.

NCBI and Uniprot Product Information

• NCBI GI #: 4501859
• NCBI GeneID: 36
• NCBI Accession #: NP_001600
• NCBI GenBank Nucleotide #: NM_001609.3
• UniProt Accession #: P45954; Q5SQN6; Q96CX7; B4DQ51
• Molecular Weight: 46.4 kDa (424aa)
• NCBI Official Full Name: short/branched chain specific acyl-CoA dehydrogenase, mitochondrial isoform 1
• NCBI Official Synonym Full Names: acyl-CoA dehydrogenase short/branched chain
• NCBI Official Symbol: ACADSB
• NCBI Official Synonym Symbols: ACAD7; SBCAD; 2-MEBCAD
• NCBI Protein Information: short/branched chain specific acyl-CoA dehydrogenase, mitochondrial
• UniProt Protein Name: Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial
• Protein Family: Short/branched chain specific acyl-CoA dehydrogenase
• UniProt Gene Name: ACADSB
• UniProt Synonym Gene Names: SBCAD; 2-MEBCAD; 2-methylbutyryl-CoA dehydrogenase

NCBI Description

Short/branched chain acyl-CoA dehydrogenase(ACADSB) is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. Substrate specificity is the primary characteristic used to define members of this gene family. The ACADSB gene product has the greatest activity towards the short branched chain acyl-CoA derivative, (S)-2-methylbutyryl-CoA, but also reacts significantly with other 2-methyl branched chain substrates and with short straight chain acyl-CoAs. The cDNA encodes for a mitochondrial precursor protein which is cleaved upon mitochondrial import and predicted to yield a mature peptide of approximately 43.7-KDa. [provided by RefSeq, Jul 2008]

Uniprot Description

Has greatest activity toward short branched chain acyl-CoA derivative such as (s)-2-methylbutyryl-CoA, isobutyryl-CoA, and 2-methylhexanoyl-CoA as well as toward short straight chain acyl-CoAs such as butyryl-CoA and hexanoyl-CoA. Can use valproyl-CoA as substrate and may play a role in controlling the metabolic flux of valproic acid in the development of toxicity of this agent.