Rabbit Calnexin Polyclonal Antibody | anti-Calnexin antibody

Calnexin (Phospho-S583) Polyclonal Antibody

Cat. Num. AAA3004929

• Gene Names: CANX; CNX; P90; IP90
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Calnexin; Polyclonal Antibody; Calnexin (Phospho-S583) Polyclonal Antibody; IP90; Major histocompatibility complex class I antigen-binding protein p88; p90; CANX; anti-Calnexin antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-Calnexin (S583) polyclonal antibody detects endogenous levels of Calnexin protein only when phosphorylated at Ser583
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 592

• Applicable Applications for anti-Calnexin antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB: 1:10,000-1:50,000; IHC: 1:50-1:1000; IP: 1:50-1:200
• Immunogen: Synthetic phosphopeptide derived from human Calnexin around the phosphorylation site of Serine 583.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Calnexin (phospho-S583) pAb at 1:10000 to 1:50000 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:SGC7901 whole cell lysate (40ug) Lane3:A375 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of p-Calnexin (S583) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Immunoprecipitation (IP)

(Immunoprecipitation of L02 whole cell lysate using Calnexin (phospho-S583) pAb (Sepharose Bead Conjugate) (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) (lane 4 and lane 5 ). Lane 1 is 20% input. The Western Blot was probed using Calnexin (phospho-S583) pAb # MBS3004929. “up-arrow” (supernatant) ; “down-arrow” (deposition))

Western Blot (WB)

(Western Blot (WB) analysis of Calnexin (phospho-S583) pAb at 1:5000 dilutionLane1:A375 whole cell lysate (40ug) Lane2:The Lung tissue lysate of Mouse (40ug) Lane3:PC12 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug) Lane5:SGC7901 whole cell lysate (40ug))

Western Blot (WB)

(Western Blot (WB) analysis of Calnexin (phospho-S583) pAb at 1:20000 dilutionLane1:L02 whole cell lysateLane2:HEK293T incubated at 42ºC for 1 hour whole cell lysateLane3:HEK293T whole cell lysateLane4:SGC7901 whole cell lysateLane5:A375 whole cell lysate)

Related Product Information for anti-Calnexin antibody

Calnexin and Calregulin (also called Calreticulin) are calcium-binding proteins that are localized to the endoplasmic reticulum—Calnexin to the membrane and Calregulin to the lumen. Calnexin is a type I membrane protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may play a role in assisting with protein assembly and in retaining unassembled protein subunits in the endoplasmic reticulum. Calregulin has both low- and high-affinity calcium-binding sites. Neither Calnexin nor Calregulin contains the calcium-binding "E-F hand" motif found in calmodulins. Calnexin and Calregulin are important for the maturation of glycoproteins in the endoplasmic reticulum and appear to bind many of the same proteins.

NCBI and Uniprot Product Information

• NCBI GI #: 66933005
• NCBI GeneID: 821
• NCBI Accession #: NP_001019820.1
• NCBI GenBank Nucleotide #: NP_001019820.1
• UniProt Accession #: P27824
• Molecular Weight: ~ 90kDa
• NCBI Official Full Name: calnexin isoform d
• NCBI Official Synonym Full Names: calnexin
• NCBI Official Symbol: CANX
• NCBI Official Synonym Symbols: CNX; P90; IP90
• NCBI Protein Information: calnexin
• UniProt Protein Name: Calnexin
• Protein Family: Calnexin
• UniProt Gene Name: CANX
• UniProt Entry Name: CALX_HUMAN

NCBI Description

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2018]

Uniprot Description

Calnexin: a calcium-binding protein of the calreticulin family. A type I membrane protein of the endoplasmic reticulum .Interacts with newly synthesized glycoproteins in the endoplasmic reticulum. May act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins.

Protein type: Endoplasmic reticulum; Calcium-binding; Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: dendrite cytoplasm; endoplasmic reticulum membrane; smooth endoplasmic reticulum; protein complex; rough endoplasmic reticulum; endoplasmic reticulum; endoplasmic reticulum lumen; dendritic spine; cell soma; membrane; axon; melanosome; ribosome

Molecular Function: ionotropic glutamate receptor binding; protein binding; unfolded protein binding; apolipoprotein binding; calcium ion binding; glycoprotein binding; carbohydrate binding

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; cellular protein metabolic process; protein folding; synaptic vesicle endocytosis; protein secretion; antigen processing and presentation of exogenous peptide antigen via MHC class II; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; aging