Reactivity Chart
Goat ATG12 Polyclonal Antibody | anti-ATG12 antibody
ATG12 Polyclonal Antibody
Immunofluorescence: 1:50-1:250
Store at -20°C for long term.
Avoid freeze/thaw cycles.
Reactivity Chart
Reactivity Chart
Testing Data
Testing Data
Immunofluorescence (IF)
Immunofluorescence (IF)
Goat polyclonal antibody to ATG12 - autophagosome marker. Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy.
NCBI and Uniprot Product Information
NCBI Description
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008]
Uniprot Description
Function: Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through an ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. The ATG12-ATG5 conjugate also regulates negatively the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Plays also a role in translation or delivery of incoming viral RNA to the translation apparatus. Ref.5 Ref.9 Ref.11 Ref.12 Ref.14 Ref.16 Ref.19
Subunit structure: Forms a conjugate with ATG5. The ATG12-ATG5 conjugate forms a complex with several units of ATG16L. Interacts with ATG3, ATG7 and ATG10. ATG12-ATG5 also interacts with MAVS, MGA, RARRES3 and TECPR1. Ref.4 Ref.5 Ref.6 Ref.9 Ref.18 Ref.19
Subcellular location: Cytoplasm
By similarity. Preautophagosomal structure membrane; Peripheral membrane protein. Note: TECPR1 recruits the ATG12-ATG5 conjugate to the autolysosomal membrane. Ref.18
Tissue specificity: Ubiquitous. Ref.1
Induction: Expression is induced by mitochondrial DNA deletions, chloramphenicol and nicotinamide. Ref.8 Ref.13
Domain: Shares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage. Ref.10
Post-translational modification: Acetylated by EP300. Ref.15
Miscellaneous: Small amount of ATG5-ATG12 conjugate is enough to perform normal autophagy.
Sequence similarities: Belongs to the ATG12 family.
Sequence caution: The sequence AAH12266.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
Research Articles on ATG12
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Product Notes
The ATG12 atg12 (Catalog #AAA448066) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The ATG12 Polyclonal Antibody reacts with Rat, mouse, canine and monkey proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's ATG12 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence. Western Blot: 1:250-1:2,000 Immunofluorescence: 1:50-1:250. Researchers should empirically determine the suitability of the ATG12 atg12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATG12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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